HTRF 96-well low volume white plate

All the benefits of HTRF with ELISA plate ease of use

These plates have been specifically designed by Cisbio as an alternative to 384-well lv plate for small sample series or simply to try HTRF in a convenient environment.
Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells.
These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy.

 

Check our 384-w & 1536-w microplate recommendations

 

Plate characteristics

Format 96 well
Color White
Bottom Opaque
Maximum volume / well 40µl
Recommended working volume / well 20-30µl
Culture NOT culture treated
Lid Compatible
Sealer Compatible (and recommended)
Reader settings 96 half-area (except Envision)
Validation Inter-batch & intra-batch

HTRF 96-well low volume white plate characteristics plate

HTRF 96-well low volume white plate characteristics plate

HTRF 96 well low volume white plate characteristics plate side view

HTRF 96-well low volume white plate characteristics plate

Plate validation

1. cAMP cell based assay in 384 well and Cisbio 96 well plates

5 μL of HEK293 cells expressing the β2 adrenergic receptor were dispensed side-by-side in an HTRF 96-well low volume plate and in a 384-well low volume plate. Cells were stimulated for 45 min at 37°C with 5 μL of isoproterenol agonist, then 5 μL of cAMP-d2 and 5 μL of anti cAMP- cryptate from the HTRF cAMP Gs Dynamic kit were added. HTRF signal was recorded after a 1 hour incubation at RT.

S/B
Cells/w 1000 2000 3000; 4000
384 well lv plate 2.1 2.8 3.2 3.4
HTRF 96 well lv plate 2.1 2.5 2.9 3.4

 

EC50 (nM)
Cells/w 1000 2000 3000; 4000
384 well lv plate 4.4 3.6 2.9 2.4
HTRF 96 well lv plate 6.7 4.8 3.3 3.0

 

2. ERK phosphorylation assay

Cells were grown in a T175 flask at 37°C, 5% CO2 for 48 h. They were then stimulated with 50 nM EGF for 5 min. After medium removal, the cells were lysed with 3 mL of supplemented lysis buffer #1 for 30 min at room temperature. Soluble fractions were collected after 10 min centrifugation. Serial dilutions of the cell lysate were performed in the supplemented lysis buffer #1.

16 µL of each dilution were dispensed for side-by-side comparison in a 384-w low-volume plate and in the HTRF 96-w low-volume. 4 µL of HTRF advanced phospho-ERK (Thr202/204) detection reagents were then dispensed and the HTRF signal was recorded after a 4 H incubation at room temperature.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
HTRF 96 well low volume plate66PL96001
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HTRF 96 well low volume plate - 5 plates66PL96005
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HTRF 96 well low volume plate - 25 plates66PL96025
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HTRF 96 well low volume plate - 100 plates66PL96100
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