Phospho-ZAP-70 (Tyr319) for monitoring T-lymphocyte activation
This cell-based assay is designed to monitor the modulation of phosphorylated ZAP-70 (Tyr319) which is a readout of T cell activation.
ZAP-70 is a cytoplasmic protein tyrosine kinase expressed in T and NK cells, and which plays a critical role in the events involved in initiating T-cell responses by the antigen receptor. Following TCR engagement, Zap-70 is rapidly and transiently phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the tyrosine kinase Lck. Increased Zap-70 kinase activity mediates downstream signaling events such as the linker for the activation of T cells (LAT) and the 76 kDa SH2-domain-containing leukocyte protein (SLP-76), which are subsequently phosphorylated by active ZAP-70. The consequences of these early signaling events eventually lead to T-cell activation, proliferation, and differentiation.