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Human S100A12 protein (Calgranulin C, EN-RAGE) in serum - ELISA kit Inflamark

Human S100A12 protein (Calgranulin C, EN-RAGE) in serum - ELISA kit Inflamark

ELISA kit for serum S100A12

The S-INFL-ELISA kit is designed to measure human S100A12 protein in serum. This kit is available for research use only. S100A12 protein is over-expressed at inflammatory sites and its serum concentrations are correlated with disease stage and activity. It is abundant in the bowel mucous of patients affected by inflammatory bowel disease, including Crohn’s disease and ulcerative colitis.

Features

  • Working range: 0 – 650 ng/mL
  • Sample: serum (obligatory 1/11predilution) – 30 µL
  • Analytical sensitivity: 21 ng/mL
  • Functional sensitivity: -

Clinical interest

  • Clinical investigation of patients with Crohn’s disease
  • Clinical investigation of patients with ulcerative colitis
  • Non-invasive assessment of intestinal damage in subjects with ulcerative colitis

ELISA

Microplate

A first monoclonal antibody, immobilized on the microplate, captures the S100A12 proteins contained in the calibrators and samples. After washing, the absorbed proteins are then recognized by a second monoclonal antibody conjugated to HRP (Horse-Radish-Peroxidase). A 1/11 sample predilution is required. 3 step protocol: 16h at RT + 2h at RT + 10 min at RT.

Sample n Median
ng/mL
95% of values obtained
ng/mL
Sera 98 55 20 -250




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