HTRF 2013 symposium

5th Symposium
HTRF in Drug Discovery
Avignon, France – April 24th-26th, 2013

Look back on the conference or see what you missed…

Our cutting edge, scientific program helped to provide new depths of insight for the application of HTRF technology to symposium attendees. Speakers from academia and industry led an engaging series of topics.
View symposium posters and presentations from the best and brightest in the field of drug discovery working to shape the future of pharmaceutical sciences, in the tabs below.

They support the HTRF symposium.

BMG Labtech Berthold Technologies Biotek CCS Cell Culture ServiceCyBio GNF Systems Greiner bio-one Molecular devices Tecan

 

Testimonials

What they said about the event

The HTRF symposium was a great opportunity to meat the representatives from Cisbio, to connect with peers from industry and academia, and all that in a great venue. The city, the sun, the science and the nice people made it an energizing conference. Thank you for the organization.

Ineke Fonteyn - Janssen Pharmaceutical - Belgium

The htrf symposium facilitates interdisciplinary exchange and networking as the common link of all the excellent science was not a certain target class or disease area but a general tool and its wide range of applications.

Oliver Wehmeier - CCS Cell Culture Service - Germany

Very well organized meeting in a beautiful place with a complete and up to date review of the HTRF applications.

Denis Servent - CEA - France

It was a good blend of learning, networking and fun. Cisbio does a fabulous job of getting like minded people together.

Michelle Mays - Boehringer Ingelheim - USA

Talks

Please click on the titles to have access to the presentations

Wednesday 24th Opening
3:00 PM Registration  
4:30 PM Welcome address François Degorce,
Director, Strategic Marketing & Corporate Communication
Cisbio Bioassays - France
5:00 PM keynote HTS: A Technology In Transition Jeff Paslay, Consultant
Paslay Consulting Group - Seattle, WA, USA
6:00 PM Tanycytes as gatekeepers of the Metabolic Brain Vincent Prévot , Scientist & Group Leader
Development and Plasticity of the Postnatal Brain Inserm Research Team, France
7:30 PM Welcome reception - Cours du Châtelet Sponsored by
BMG Labtech
Thursday 25th Revisiting GPCR and receptor screening
7:45 AM Registration  
8:30 AM HTRF Technology introduction Glenn Knapik, Vice President
Cisbio Bioassays - USA
8:40 AM Session intro Rochdi Bouhelal (chair), Senior Investigator I,
Center for Proteomic Chemistry, Novartis Institutes for Biomedical Research, Switzerland
8:55 AM Applications of HTRF and Tag-lite Assays for HTP Antibody Screening Brigitte Devaux, Director, Hybridoma Research Department
Bristol Myers Squibb - CA, USA
9:25 AM Evaluation of a Tag-lite binding assay for a class B receptor Andreas Kahrs, Principal Scientist,
Lead Identification & Optimization Support - Boehringer Ingelheim - Germany
9:55 AM Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery Katrin Nowak-Reppel, Scientist
Screening Department - Bayer Healthcare - Germany
10:25 AM Poster snapshot #1  
10:30 AM Break Sponsored by
CyBio
11:00 AM A Novel HTRF assay to measure the conformational change in the extracellular domain of a class C GPCR Eric Johnson, Research Fellow
Automated Biotechnology - Merck Sharp & Dohme - NC, USA
11:30 AM keynote Structure, function and pharmacology of G protein-coupled receptors - state-of-the-art and future challenges Hans Bräuner Osborne, Professor
Molecular Pharmacology - University of Copenhagen - Denmark
12:30 PM Lunch Sponsored by
Berthold Tecnnologies
  Biomarkers, signaling pathways and functional readouts
2:00 PM Session intro Karsten Parczyk (chair), Head of Screening at Lead Discovery Berlin
Bayer Healthcare - Germany
2:15 PM Identification of proteins that modulate protein synthesis activity in human skeletal muscle myotubes using a Cisbio pRPS6 assay Art Brace, Director
Discovery Biology - Five Prime Therapeutics - CA, USA
2:45 PM Use of phenotypic assays in high throughput screening campaigns to enable novel drug discovery John Joslin, Research Investigator
Genomics Institute of the Novartis Research Foundation - CA, USA
4:00 PM Poster snapshot #2  
4:05 PM Break Sponsored by
GNF Systems
4:35 PM Cell-based kinase assays in HTS – potential and limitations for primary and secondary screening Benjamin Bader, Senior Scientist & Group Leader,
Screening Department - Bayer Healthcare - Germany
5:05 PM Multifaceted use of HTRF technology in pre-clinical drug development Patrick Sarmiere, Scientist
Pre-Clinical - Accorda Therapeutics - NY, USA
5:35 PM Cellular high throughput screening assays using HTRF detection of cytokines Lorena Kallal, Manager
Biological Reagents and Assay Development - GlaxoSmithKline - PA, USA
7:30 PM Conference banquet - Salle Grand Tinel at the Pope's Palace Sponsored by
BMG Labtech
Friday 26th Emerging new targets and screening tools
8:30 AM Session intro Doris Hafenbradl (chair), Vice President, Discovery Services
Axxam - Italy
8:45 AM The Use of HTRF in Biologics Discovery Liz England, Scientist
Medimmune - UK
9:15 AM Real time internalization of incretin receptors Maria Waldhoer and Nikolaj Kulahin, Research scientists
NovoNordisk - Denmark
9:45 AM HTRF technology: a robust and versatile assay platform for measuring biological interactions Zhao Ren, Associate Director, Assay Development and Screening group
Elan Pharmaceuticals - CA, USA
10:15 AM Poster snapshot #3  
10:20 AM Break Sponsored by
Tecan
10:50 AM High-throughput assay development for antibody therapeutics Pamela Chan, Scientific Manager,
Genentech - CA, USA
11:20 AM keynote Drug discovery on an epigenetic target class: protein methyl transferase inhibitors as personalized cancer therapeutics Margaret Porter Scott, Director of Lead Discovery,
Epizyme - USA
12:20 PM Poster snapshot #4  
12:25 PM Lunch box with poster session Sponsored by
BMG Labtech
1:30 PM HTRF-powered detection of ubiquitin-like protein (UBL) signaling and interactions Eduard A. Sergienko, Director
Assay Development - Sanford-Burnham Medical Research Institute - CA, USA
2:00 PM HTRF in bioanalytical method development Esohe Idusogie, Associate Director
Process Development Analytical - Oncomed
  How can pharma and academia partner for drug screening?
2:30 PM Session intro Ahmad Kamal (chair), Team Leader
Cellular Pharmacology - MRC Technology - UK
2:45 PM Translating academic research into early stage drug discovery projects Sheraz Gul, Vice President, Head of Biology
European Screening Port - Germany
3:15 PM Break Sponsored by
Molecular Devices
3:45 PM Can HTRF technology answer to different scientific questions ? An academic with industrial standards point of view Pascal Villa, Director
Plate-forme de Chimie Biologique Intégrative de Strasbourg - Université de Strasbourg - France
4:15 PM Experience on HTRF technology in public-private collaborations Jose Manuel Brea Floriani, Professor of Pharmacology
USEF Platform, University of Santiago de Compostela - Spain
4:45 PM Access Platform: A new way to partnering Carole Fages, Manager, global research project,
Sanofi - France
5:15 PM Using HTRF to identify chemical probes against infectious diseases Franck Madoux, Senior Scientist
Scripps Institute - FL, USA
5:45 PM Symposium closure  
6:00 PM Farewell reception - Cloitre Benoit XII Sponsored by
BMG Labtech
Saturday 27th  
8:30 AM Social event  
4:00 PM End of symposium  

Posters

Please click on the titles to have access to the posters

Biotherapeutics
Use of HTRF® technology to support pre-clinical biomarker studies for the development of antibodies in oncology. Are our biotherapeutics hitting our target? Attenot F, Nicolazzi C, Chiron M, Henry C
Sanofi, Vitry-sur-Seine, France
Elisa conversion and biomarkers
Multiplexed Assay for IL-6 secretion and Cell Viability using an Epithelial Ovarian Cancer Cell Line Larson B, Banks P, Pierre N, Graham S, Jacquemart L, Degorce F
BioTek Instruments, Inc., Vermont, USA. Cisbio Bioassays, Codolet, France
The Infinite® F200 PRO – a cost- efficient and high performance HTRF® reader Oberdanner C, Fejtl M, Flatscher K
Tecan Austria GmbH, Grödig, Austria
The use of HTRF® assay to screen small molecules which inhibit the differentiation of T helper 17 (Th-17) cells Zhang F, Xie X
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China
A New HTRF® cell-based assay for the Quantification of Active GLP-1 Vallaghé J, Bdioui S, Mensat P, Martinez S, Degorce F, Trinquet E, Tokuda C, Adachi K
Cisbio Bioassays, Codolet, France. Sceti Medical Labo K.K., Reagents Section, Tokyo, Japan
HTRF® - A Beneficial Tool for Lead Optimization of Biotherapeutics Mays M, Gupta P, Chlewicki L, Canada
Boehringer Ingelheim
Epigenetics
High-throughput homogeneous histone methyltransferase and demethylase assays using HTRF® technology and the SpectraMax® Paradigm® microplate detection platform Olsen C, Pierre N, Gonzalez-Moya A, Graham S, Degorce F
Molecular Devices, Sunnyvale, CA . Cisbio US, Bedford, MA, USA
Development of a homogeneous time-resolved fluorescence assay to identify inhibitors of CREB Binding Protein Stock I, Sahasrabudhe P, Loria P, Pletcher M
Pfizer
High-Throughput Homogeneous Histone H3 Methyltransferase (HMT) and Demethylase (HDMT) Enzyme Assays using HTRF Technology: G9a H3K27 dimethylation assay example Roux T, Adachi K, Tokuda C, Verdi J, Junique S, Trinquet E, Gonzalez-Moya A, Degorce F
Sceti Medical Labo K.K., Tokyo, Japan. Cisbio Bioassays, Codolet , France. Cisbio US, Bedford, MA.
GPCRs
Evaluation of the Cisbio Cellul’erk HTRF® kit as a tool to investigate receptor pharmacology Sattikar AM, Rosethorne EM , Kent T, Dowling RM
Novartis Institutes for Biomedical Research, Horsham U.K.
Time-resolved FRET microscopy to study GPCR oligomerization and receptor internalization in cells and native tissues Falco A, Faklaris O, Cottet M, Laget M, Trinquet E, Pin JP, Mouillac B, Durroux T
Institut of Functional Genomics (IGF), Montpellier, France. Cisbio Bioassays, Codolet, France
Low Affinity Inhibitors of the Inositol Phosphate (IP) Signaling Pathway Found Using the PHERAstar FS and the IP-One HTRF® Assay Dell EJ, Orban M, Tardieu JL, Degorce F
BMG LABTECH GmbH, Ortenberg, Germany. Cisbio Bioassays, Codolet, France
Characterisation of Inositol Phosphate and cAMP Signalling from GPCRs using Homogeneous Time-Resolved Fluorescence Technology Johnstone E KM, Armstrong S, Akli Ayoub M, Pfleger K DG
University of Western Australia, Australia. College of Science, King Saud University, Kingdom of Saudi Arabia.
Metabotropic glutamate receptors FRET sensors as new tools for high-throughput drug screening and profiling Fabre L, Scholler P, Doumazane P, Donsimoni G, Rovira X, Roux T, Jean A, Zwier JM, Trinquet E, Rondard P, Pin JP.
Institut of Functional Genomics (IGF), Montpellier, France. Cisbio Bioassays, Codolet, France
HTRF® Europium and Terbium Cryptate Assays on the New SpectraMax® i3 Multi-Mode Microplate Platform Monnet L, Olsen C, Lebreton ML
Molecular Devices, Sunnyvale, USA
D2-5-HT1A receptor heteromers as a new target for antipsychotic drug delivery Łukasiewicz S, Błasiak E, Szczepanowicz K, Dziedzicka-Wasylewska M
Jagiellonian University, Krakow, Poland. Institute of Catalysis and Surface Chemistry PAS, Krakow, Poland
Tools Implementation for the discovery and characterization of GPCR agonists biased or pathways selective Kiry M, Romier M, Jaga D, Leroy X
Actelion Pharmaceuticals Ltd., Allschwil, Switzerland. Cisbio Bioassays, Codolet, France.
Structure-activity characterizations of glucagon receptor, an example of using HTRF® cAMP assay He M, Yang D, Zhang Z, Zhou C, Cai X, Wang J, Feng Y, Deng J, Dai A and Wang M
Shanghai Institute of Materia Medica, Chinese Academy of Sciences (CAS), Shanghai, China
Sensing conformational dynamics of the GABAB receptor: a new tool for drug discovery Lecat-Guillet N, Scholler P, Monnier C, Stewart G, Kniazeff J, Zwier JM, Trinquet E, Rondard P, Pin JP
Institut of Functional Genomics (IGF), Montpellier, France. Cisbio Bioassays, Codolet, France
Sensing conformational dynamics of metabotropic glutamate receptors Scholler P, Fabre L, Doumazane E, Donsimoni G, Roux T, Zwier JM, Trinquet E, Rondard P, Pin JP
Institut of Functional Genomics (IGF), Montpellier, France. Cisbio Bioassays, Codolet, France
Ex vivo IP1 Determination using Cisbio’s HTRF® IP-One assay in Rodent Brain extracts after Muscarinic M1 receptor activation Smets T, de Waepenaert K, Hens K, Vanhoof G
Janssen Pharmaceutical Companies of Johnson & Johnson, Beerse, Belgium
Activation mechanism of GABAB receptor through dynamic Protein–protein interactions Zhang W, Tu H, Xu C, Wang Y, Sun Q, Liu J
Sino-France Laboratory of Cellular Signaling, Huazhong University of Science and Technology, Wuhan, Hubei, China
Biomimetic Screening of Class-B G Protein-Coupled Receptors Cuboni S, Kozany C, Devigny C, Hoogeland B, Hausch F
Max Planck Institute of Psychiatry, Munich, Germany
GPCRs and Tag-lite®
Profiling of GPCR targeting drugs using the Tag-lite® technology Belhocine A, Veglianese P, Hounsou C, Dupuis E, Durroux T, Goudet C, Trinquet E and Pin JP
Institut of Functional Genomics (IGF), Team Cell2Lead, Montpellier, France. Cisbio Bioassays, Codolet, France. Dulbecco Telethon Institute, Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy.
One Acceptor to rule them all: fluorescent Asenapine derivatives as "universal" acceptor ligand for Tag-lite® binding assays

Hounsou C, Belhocine A, Rodriguez T, Durroux T, Pin JP, Dupuis E, Roux T, Lamarque L, Gasparik V, Bonnet D and Hibert M
Institut of Functional Genomics (IGF), Montpellier, France. Cisbio Bioassays, Codolet, France. Laboratoire d'Innovation Thérapeutique (LIT), UMR7200 Université Strasbourg

Cell-based assays using Tag-lite® technology for ligand-gated ion channel investigations: Example of the 5HT3-A serotonin receptor Dupuis E, Blanc E, Mathis G, Wagner P, Bourguignon J-J, Hibert M
Cisbio Bioassays, Codolet, France . Laboratoire d’Innovation Thérapeutique (LIT), Université Strasbourg, France
An innovative specific Dopamine D3 fluorescent ligand for D1R-D3R heteromers investigation using HTRF® technology Oueslati N, Belhocine A, Margathe JF, Hibert M, Dupuis E, Mathis G, Hounsou C , Durroux T and Bonnet D
Institut of Functional Genomics (IGF), Montpellier, France. Cisbio Bioassays, Codolet, France.
Pharmacological characterization of Ghrelin receptor agonists in functional and Tag-lite® ligand binding assays

Ferrand S, Mathis M, Reymann N, Schopfer U, Bouhelal R
Novartis Institutes for Biomedical Research, Horsham U.K.

Selective Fluorescent Non-peptidic Antagonists For Vasopressin V2 GPCR: Application To Ligand Screening and Oligomerization Assays Bonnet D, Loison S, Cottet M, Orcel H, Lamarque L, Trinquet E, Dupuis E, Mouillac B, Hibert M, Durroux T
Laboratoire d’Innovation Thérapeutique, Faculté de Pharmacie, Illkirch, France. Institut of Functional Genomics (IGF), Montpellier, France. Cisbio Bioassays, Codolet, France.
Fluorescent non-peptidic antagonists for V1a-R: Application to Tag-lite® technology and GPCR oligomerization assays using HTRF® Rodriguez T, Pflimlin E, Ferrasse A, Hounsou C, Loison S, Wolf E, Lamarque L, Dupuis E, Hibert M, Durroux T, Bonnet D
Laboratoire d’Innovation Thérapeutique, Faculté de Pharmacie, Illkirch, France. Institut of Functional Genomics (IGF), Montpellier, France. Cisbio Bioassays, Codolet, France.
Tag-lite® technology: adding a new level to drug discovery analysis Wittig V, Grau-Richards S, Jaeger C
Roche Glycart AG, Schlieren, Switzerland
Cross-talk between GPCRs in homodimeric assembly analyzed with Tag-lite® technology Li X, Rovira X, Scholler P, Liu J, Pin J.P, Rondard P
Institut of Functional Genomics (IGF), Montpellier, France. Sino-france lab of cellular signaling, College of Life Sciences, Huazhong University of Science and Technology, Wuhan, China.
Implementation of Cisbio’s HTRF® M1 muscarinic receptor related assays on CyBio liquid handling solutions for small, medium and high throughput Jean A, Tardieu JL, M. Préaudat M, Degorce F, Undisz K
Cisbio Bioassays, Codolet, France. Cybio AG, Jena, Germany
Industry-academia partnerships
An advanced platform for receptor biology and pharmacolology investigation Maurel D, Brabet I, Vol C, Prézeau L
Institut of Functional Genomics (IGF), Montpellier, France
Kinases
An Automated, High Throughput Method for the Quantification of Endogenous Cellular Kinase Activity Larson B, Banks P, Pierre N, Graham S, Jacquemart L, Degorce F
BioTek Instruments, Inc., Vermont, USA. Cisbio Bioassays, Codolet, France.
Take advantages of HTRF® to simplify HTS Cascade Zheng Z, Shao Y, Liu J
Innovation Center China, AstraZeneca, Shanghai, China
Do all Cell-based Kinase Assays Perform Similarly? A Side-by-side Comparison of HTRF®, Western Blot, Elisa and AlphaScreen® Surefire® Charrier-Savournin F, Vallaghé J, Costy N, Douzon S, Chouvet C, Boisseau C, Degorce F, Trinquet E
Cisbio Bioassays, Codolet, France
New HTRF® Normalization cell-based kinase kits: HTRF® Total ERK, Total Stat3 and Total MEK1 assays to support exploration of cell signaling pathways Charrier-Savournin F, Chouvet C, Vallaghé J, Degorce F, Trinquet E
Cisbio Bioassays, Codolet, France
Protein:proteins interactions
An original approach to detect phospho-S/T containing peptides by combining chemical transformation and HTRF® technology Alpha-Bazin B, Höcht P, Quéméneur E
CEA, DSV, IBEB, Service de biochimie et toxicologie nucléaire, France
Analysis of the glycoproteins interactions involved in the HCMV core fusion machinery using time-resolved FRET Comps-Agrar L, Fouts A, Stengel K, Auerbach M, Lee M, Eaton D and Feierbach B
Genentech, CA, USA