HTRF® microplate recommendations

Most available microplate brands are compatible with HTRF technology and products. Nevertheless, background levels may differ substantially among various plate types. FIA white plates from many suppliers remain the most appropriate standard. Tissue-culture treated, or surface-treated plates may also be used. Glass bottom plates produce suboptimal results and should not be used. The following plates produced excellent results during our validation process for a broad range of HTRF assays.

 

Check our HTRF 96-well low volume plate

 

Provider Format Recommended volume Type Cat#
Cisbio 96-w low volume 20-30 µL White 66PL96001
Greiner 384-w 80-100 µL White 781075
Greiner 384-w low volume 15-20 µL White 784075
Greiner 384-w 80-100 µL White - TC* - sterile 781073
Greiner 384-w 80-100 µL White - TC* - sterile 781080
Greiner 384-w low volume 15-20 µL White - TC* - sterile 784080
Greiner 1536-w 5-10 µL White 783075
Greiner 1536-w 5-10 µL White - TC* - sterile 782080
Greiner 1536-w 5-10 µL White - TC* - sterile 782078
Greiner 1536-w 5-10 µL White, sterile 782073
Greiner 1536-w 5-10 µL White - TC* - sterile 782093
NUNC 384-w low volume 15-20 µL White, NT 264706

Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery. For instance, when assay volumes are equal to or lower than 20 µl, it is better to use 384 low-volume or 1536-well plates rather than regular 384-well plates.

The signal to noise ratios obtained using white microplates do not differ greatly from those of black plates. Absolute counts, however, are significantly higher in white plates, thereby improving count statistics and subsequent assay reproducibility and sensitivity. NOTE: The use of white plates is mandatory for some assays, such as IP-One, and highly recommended when detection is performed using certain instruments, in particular on monochromator-based plate readers.