HTRF® cell-based assay for quantifying phosphorylated IRF3
Cisbio's cell-based homogeneous HTRF® phospho-IRF3 (Ser385/386) immunoassay enables the quantitative detection of IRF3 phosphorylated on Serine 385/386. In viral infected cells, the phosphorylation of transcription factor IRF3 induces the production of antiviral and proinflammatory cytokines, a key element in the innate immunity system. Using a streamlined protocol without any washing steps and amenable to low-volume formats, this phospho-IRF3 assay can be used from basic research through to preclinical drug discovery phases. The kit contains all the reagents you need, and offers increased throughput compared to ELISA or Western Blot.