6His Check kit Gold HTRF®
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No-wash
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Ready-to-use
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Ease-of-use
Overview
The kit enables the rapid detection of 6His tagged fusion proteins at each step of protein production and purification. It can either be used to ensure that the 6His motif of a fusion protein is accessible to the anti-6His antibody, or to approximate the concentration of a 6His-tagged protein.
Calibrated using the appropriate protein, the assay is very accurate and compatible with the SOPs, it can also be used to eliminate false positives and to predict the possibility of purifying the protein using a Nickel column: a non-detected Tag using our check kit will not be available for purification.
Benefits
- ELIMINATION OF FALSE POSITIVES
- PROTEIN QUANTIFICATION
- TAG ACCESSIBILITY
Assay principle
Assay protocol
6His Check Kit Gold performance on various 6His-tagged proteins
The 6His check kit Gold enables the detection of 6His tagged proteins, whatever the molecular weight and the 6His tag insertion (N-terminal, C-terminal and internal 6His tags). The detection of the 6His tag varies for different fusion proteins.
For quantitative determination of 6His tagged protein in a sample, we recommended using an internal calibrator (6His fusion protein close to the 6His tagged protein of interest).
*The 6His-MBP-GST-Flag and the THX-6His-1509V3 proteins were kindly provided by Hybrigenics.
| Tag insertion | 6His Tagged protein | MW(Da) | Quantification limit or IC10(nM) | IC50 (nM) |
|---|---|---|---|---|
| N-term | 6His-peptide | 1,300 | 4.2 | 32.8 |
| 6His BRD4(1) | 17,800 | 17.6 | 105.0 | |
| 6His-SNAP | 20,600 | 31.4 | 244.8 | |
| 6His-MBP-GST-Flag* | 64,000 | 3.2 | 28.0 | |
| Internal | THX-6His-1509V3* | 27,000 | 3.0 | 24.8 |
| C-term | 6His tagged Nanobody | 15,000 | 2.9 | 24.48 |
HTRF check kits for GST and 6His tag accessibility
Procedure and guidelines for assessing tagged proteins - Application Notes
Accelerate throughput without compromising on performance
HTRf® reagents for biotherapeutics - Flyers
Product Insert 6His Check Kit / 64HISPEH
64HISPEH - Product Insert
Product Insert 6His Check Kit / 64HISPEG
64HISPEG - Product Insert
HTRF PPI your dream assay served on a plate
Sandwiches aren't just for eating - Infographics
Best practices for pharmacological characterization of PPI inhibitors
Easy pharmacological characterization of PPI modulators. - Technical Notes
HTRF assays handle low- to high affinity protein-protein interactions
Deciphering low- and high affinity interactions - Application Notes
Nuclear receptor ligand identification with HTRF
Monitoring nuclear receptor binding with HTRF assays - Application Notes
HTRF addresses large protein-protein interaction complexes
Challenge large complexes with HTRF assays - Application Notes
A brief history of Protein-Protein Interactions
How well do you know PPI? - Infographics
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
The ultimate guide to PPI assays with HTRF
Complete guide - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Virology research solutions using HTRF Protein-Protein Interaction assays
See how peer researchers challenge the viral life cycle with PPI assays - Application Notes
Safety Data Sheet 6His Check Kit / 64HISPEH
64HISPEH - Safety Data Sheet
Safety Data Sheet (DEU) 6His Check Kit / 64HISPEH
64HISPEH - Safety Data Sheet
Safety Data Sheet (ELL) 6His Check Kit / 64HISPEH
64HISPEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) 6His Check Kit / 64HISPEH
64HISPEH - Safety Data Sheet
Safety Data Sheet (ITA) 6His Check Kit / 64HISPEH
64HISPEH - Safety Data Sheet
Safety Data Sheet (SPA) 6His Check Kit / 64HISPEH
64HISPEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) 6His Check Kit / 64HISPEH
64HISPEH - Safety Data Sheet
Safety Data Sheet (ENG-US) 6His Check Kit / 64HISPEH
64HISPEH - Safety Data Sheet
Safety Data Sheet (DEU) 6His Check Kit / 64HISPEG
64HISPEG - Safety Data Sheet
Safety Data Sheet (ELL) 6His Check Kit / 64HISPEG
64HISPEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) 6His Check Kit / 64HISPEG
64HISPEG - Safety Data Sheet
Safety Data Sheet (ITA) 6His Check Kit / 64HISPEG
64HISPEG - Safety Data Sheet
Safety Data Sheet (SPA) 6His Check Kit / 64HISPEG
64HISPEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) 6His Check Kit / 64HISPEG
64HISPEG - Safety Data Sheet
Safety Data Sheet (ENG-US) 6His Check Kit / 64HISPEG
64HISPEG - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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