cAMP Gs dynamic kit
Optimal performance when applied to Gs activation or inhibition
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Active GLP-1, also known as glucagon-like peptide-1 active forms (GLP-1(7-36)NH2, and GLP-1(7-37)), has become a key biomarker in treatment of type 2 diabetes. The main actions of GLP-1 are stimulation of insulin secretion, and inhibition of glucagon secretion and food intake. In vivo, the active forms are rapidly degraded into inactive forms (9-36)NH2 and (9-37) by the dipeptidyl peptidase IV (DPP-IV). The active GLP-1 kit is designed for a rapid detection of GLP-1 in cell supernatants.
Active GLP-1 | |
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Incubation | ON at RT |
Linear range | 43. 8 to 1,400 pg/mL |
Detection limit | 25 pg/mL* |
Species reactivity | Rat, mouse, human, porcine |
Peptide | Cross reactivity |
GLP-1 (7-36) amide | 100% |
GLP-1 (7-37) | 98.40% |
GLP-1 (1-36) amide | 0.39% |
GLP-1 (1-37) | 0.37% |
GLP-1 (9-36) amide | <0.01% |
GLP-1 (7-17) | <0.01% |
GLP-2 | <0.01% |
Glucagon | <0.01% |
Active GLP-1 standard curve was performed in a final 20µL assay volume and read on a Pherastar instrument (BMG LABTECH).
* Caution: all HTRF compatible readers but SpectraMax M5e and FlexStation reach this sensitivity criteria.
HTRF active GLP-1 | Fluorescent ELISA |
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1 assay step mix and read (no washing) - <1hrs assay time | 6 sequential steps - >4hrs assay time |
A time-course was determined for active GLP-1 secretion in human enteroendocrine NCI-H716 cells after glucose stimulation. Cells (2.105 cells/well) were seeded into 96-well culture plates pre-coated with Matrigel, and cultured for two days with DMEM 10% FBS medium. On the experiment day, cells were washed and incubated for 2 hours with increasing concentration of glucose in 100 µL of KRB supplemented with DPP IV inhibitor . At the end of glucose stimulation, 10 µL of supernatants were transferred to a 384 small volume-well plate, and assayed for active GLP-1 level as described in 'Assay protocol'. As expected, active GLP-1 was induced with Glucose dose dependence. The maximum amplitude of active GLP-1 triggered by 10% glucose versus basal production was 2.1.
A high throughput, mix and read solution for the study of metabolic diseases
In collaboration with Venenum Biodesign - Scientific Presentations
Product Insert GLP1 Kit / 62glppeg-62glppeh-62GLPPEH
62GLPPEH-62glppeg-62glppeh - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
A guide to help you in your diabetes projects
A comprehensive listing of Type II diabetes pathways - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Safety Data Sheet (DEU) GLP1 Kit / 62GLPPEG
62GLPPEG - Safety Data Sheet
Safety Data Sheet (ELL) GLP1 Kit / 62GLPPEG
62GLPPEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) GLP1 Kit / 62GLPPEG
62GLPPEG - Safety Data Sheet
Safety Data Sheet (ITA) GLP1 Kit / 62GLPPEG
62GLPPEG - Safety Data Sheet
Safety Data Sheet (SPA) GLP1 Kit / 62GLPPEG
62GLPPEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) GLP1 Kit / 62GLPPEG
62GLPPEG - Safety Data Sheet
Safety Data Sheet (ENG-US) GLP1 Kit / 62GLPPEG
62GLPPEG - Safety Data Sheet
Safety Data Sheet (DEU) GLP1 Kit / 62GLPPEH
62GLPPEH - Safety Data Sheet
Safety Data Sheet (ELL) GLP1 Kit / 62GLPPEH
62GLPPEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) GLP1 Kit / 62GLPPEH
62GLPPEH - Safety Data Sheet
Safety Data Sheet (ITA) GLP1 Kit / 62GLPPEH
62GLPPEH - Safety Data Sheet
Safety Data Sheet (SPA) GLP1 Kit / 62GLPPEH
62GLPPEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) GLP1 Kit / 62GLPPEH
62GLPPEH - Safety Data Sheet
Safety Data Sheet (ENG-US) GLP1 Kit / 62GLPPEH
62GLPPEH - Safety Data Sheet
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