SNAP tag A2B Adenosine Receptor cloned plasmid
Tag-lite plasmid cloned with a SNAP tag A2B Adenosine receptor
Tag-lite cells transiently expressing the A2B receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
A2B receptor is one of the four adenosine receptor family (including A1, A2A, and A3 receptors). A2B is involved in processes such as inflammation, immune responses, vasodilation, cell growth, intestinal function, and neurosecretion. Thus, it is considered as an interesting target in the treatment of cancer, diabetes, renal disease, and vascular disease.
Cells expressing the A2B receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.
Running your A2B receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells (C1T1A2B) into each well, followed by 5 µL of labeled ligand (L0068RED) and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells, and then incubated to equilibrium. The HTRF Ratio obtained from this titration is the total binding.
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Examples of data obtained using Adenosine A2B receptor labeled cells and their matching fluorescent ligand (L0068RED). XAC (Xanthine amine congener) was used for Ki determination in the competitive binding assay. Results may vary from one HTRF® compatible reader to another.
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