SNAP tag A2B AdenosineR cloned plasmid
Tag-lite plasmid cloned with a SNAP tag A2B Adenosine receptor
Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.
This Adenosine A2B receptor red antagonist is a XAC derivative labeled with a red emitting HTRF fluorescent probe.
This ligand is best suited for use in the Adenosine A2B receptor binding assay.
Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells (C1TT1A2B) into each well, followed by 5 µL of labeled ligand (L0068RED) and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells, and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
In collaboration with Boehringer Ingelheim - Scientific Presentations
In collaboration with Bayer - Scientific Presentations
Challenge the limits of binding kinetics studies - Application Notes
Binding Assays to measure therapeutic antitumor antibodies - Posters
The gold standard technology for receptor binding studies - Videos
Case study about kinetic binding characterization provided by the combination of Cisbio and Excellerate bioscience expertise. - Application Notes
Get the brochure about technology comparison. - Brochures
Available On-demand - Videos
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.Let's find your reader