SNAP tag A2B AdenosineR cloned plasmid
Tag-lite plasmid cloned with a SNAP tag A2B Adenosine receptor
Adenosine A2BR fluorescent probe HTRF®
This Adenosine A2B receptor red antagonist is a XAC derivative labeled with a red emitting HTRF fluorescent probe.
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Non-radioactive
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No-wash
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Cell-based
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Equilibrium & Kinetic binding compatible
Overview
Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.
This Adenosine A2B receptor red antagonist is a XAC derivative labeled with a red emitting HTRF fluorescent probe.
This ligand is best suited for use in the Adenosine A2B receptor binding assay.
Benefits
- FLUORESCENTLY LABELED LIGAND
- HIGHLY STABLE
- VALIDATED IN BINDING
Assay principle
Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells (C1TT1A2B) into each well, followed by 5 µL of labeled ligand (L0068RED) and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Saturation binding (KD)
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells, and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Watch this video explaining how to run a saturation binding assay using Tag-lite.
Competitive binding (KI)
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Watch this video explaining how to run a competitive binding assay using Tag-lite.
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
Evaluation of a Tag-lite binding assay for a class B receptor
In collaboration with Boehringer Ingelheim - Scientific Presentations
Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery
In collaboration with Bayer - Scientific Presentations
The easy way to approach biology without radioactivity - Flyers
Challenge the limits of binding kinetics studies - Application Notes
HTRF, a powerful assay platform for Immune Checkpoint Studies
Binding Assays to measure therapeutic antitumor antibodies - Posters
Understanding GPCRs is the key to improved DD
In collaboration with GEN - Guides
Tag-lite, the easiest solution for studying GPCR-ligands interactions
The gold standard technology for receptor binding studies - Videos
Kinetic adenosine receptor family binding assays
Case study about kinetic binding characterization provided by the combination of Cisbio and Excellerate bioscience expertise. - Application Notes
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Webinar | Association Rate Constant: The Unsung Hero of Drug Discovery Kinetics
Available On-demand - Videos
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
A comprehensive overview of pharmacology's main ligands - Guides
GPCRs: the pathway to Discovery
Complete solutions for GPCR Drug Discovery - Brochures
Manual A2B-R red antago / L0068RED
L0068RED - Product Insert
Technical Data Sheet A2B-R red antago / L0068RED
L0068RED Batch 01A - Batch Information
Technical Data Sheet A2B-R red antago / L0068RED
L0068RED Batch 01RA - Batch Information
Safety Data Sheet (DEU) A2B-R red antago / L0068RED
L0068RED - Safety Data Sheet
Safety Data Sheet (ELL) A2B-R red antago / L0068RED
L0068RED - Safety Data Sheet
Safety Data Sheet (FRA-FR) A2B-R red antago / L0068RED
L0068RED - Safety Data Sheet
Safety Data Sheet (ITA) A2B-R red antago / L0068RED
L0068RED - Safety Data Sheet
Safety Data Sheet (SPA) A2B-R red antago / L0068RED
L0068RED - Safety Data Sheet
Safety Data Sheet (ENG-GB) A2B-R red antago / L0068RED
L0068RED - Safety Data Sheet
Safety Data Sheet (ENG-US) A2B-R red antago / L0068RED
L0068RED - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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