Alpha-Tubulin Housekeeping Cellular Kit
Alpha-Tubulin Housekeeping standard HTRF®
This standard is a spare reagent for the Alpha-Tubulin Housekeeping kit. It may be used for absolute quantification of cellular alpha-tubulin extracted with Cisbio’s lysis buffer.
-
Developed and tested to Cisbio's high quality standards
Overview
This standard provides a way to quantify the alpha tubulin contained in your sample
Benefits
- PRODUCED BY MANUFACTURER
- STRINGENT QUALITY CONTROL
Correlation with the BCA protein assay
Lysates of human and mouse cell lines were plated at different cell densities in 96-well culture plates for 5 hours at 37°C, 5% CO2. Cells were then lysed with 50 µL of supplemented lysis buffer #4 for 30 minutes at RT under gentle shaking, and 4 µL of cell lysate were transferred into a low volume white microplate. For HTRF detection, 12 µL of kit diluent were added followed by the dispensing of 4 µL of premixed detection antibodies. The HTRF signal was recorded after an overnight incubation at RT. In parallel, the concentration of proteins was determined in the same lysates using the BCA protein assay (QuantiPro™ BCA Assay Kit, Sigma-Aldrich) according to the manufacturer’s instructions. For both methods (HTRF or BCA), lysates were analyzed either neat or prediluted in the appropriate buffer in order to work within the linear range of each kit. The cell densities used for the correlations are mentioned on each graph next to each point.
Product Insert a-Tubulin housek Std / 64ATUBCDA
64ATUBCDA - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Best practices for analyzing brain samples with HTRF® phospho assays for neurosciences
Insider Tips for successful sample treatment - Technical Notes
Optimize your HTRF cell signaling assays on tissues
HTRF and WB compatible guidelines - Technical Notes
Best practices for analyzing tumor xenografts with HTRF phospho assays
Protocol for tumor xenograft analysis with HTRF - Technical Notes
Key guidelines to successful cell signaling experiments
Mastering the art of cell signaling assays optimization - Guides
Multi-tissue cellular modeling and anlysis of insulin signaling - Posters
HTRF® cell signaling platform combined with iCell® Hepatocytes
A solution for phospho-protein analysis in metabolic disorders - Posters
HTRF phospho-assays reveal subtle drug-induced effects
Detailed protocol and direct comparison with WB - Posters
A single technology for 2D cells, 3D cells, and xenograft models - Posters
PI3K/AKT/mTor translational control pathway - Posters
Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells
Analysis of a large panel of diverse biological samples and cellular models - Posters
From 2D, 3D cell cultures to xenografts: A smart HTRF platform to maximize anticancer drug discovery
One technology across all samples - Application Notes
HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts
Tumor xenograft analysis: HTRF versus Western blot - Application Notes
HTRF cell-based phospho-protein data normalization
Valuable guidelines for efficiently analyzing and interpreting results - Application Notes
HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers
Increased flexibility of phospho-assays - Application Notes
HTRF Alpha-tubulin Housekeeping kit
Properly interpret your compound effect - Application Notes
Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling
Analyse of PI3K/AKT/mTor translational control pathway - Application Notes
Cell-based kinase assays in HTS ? potential and limitations for primary and secondary screening
In collaboration with Bayer - Scientific Presentations
Unleash the potential of your phosphorylation research with HTRF
A fun video introducing you to phosphorylation assays with HTRF - Videos
How to run a cell based phospho HTRF assay
3' video to set up your Phospho assay - Videos
How to run a cell based phospho HTRF assay
What to expect at the bench - Videos
HTRF cellular phospho-protein assays
Physiologically relevant results fo fast flowing research - Flyers
Guidelines for Cell Culture and Lysis in Different Formats Prior to HTRF Detection
Seeding and lysing recommendations for a number of cell culture vessels. - Technical Notes
Assessment of drug efficacy and toxicity by combining innovative technologies
Combination of AlphaLISA®, HTRF®, or AlphaLISA® SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay - Application Notes
Methodological Aspects of Homogeneous Time-Resolved Fluorescence (HTRF)
Learn how to reduce time and sample consumption - Application Notes
Safety Data Sheet (DEU) a-Tubulin housek Std / 64ATUBCDA
64ATUBCDA - Safety Data Sheet
Safety Data Sheet (ELL) a-Tubulin housek Std / 64ATUBCDA
64ATUBCDA - Safety Data Sheet
Safety Data Sheet (FRA-FR) a-Tubulin housek Std / 64ATUBCDA
64ATUBCDA - Safety Data Sheet
Safety Data Sheet (ITA) a-Tubulin housek Std / 64ATUBCDA
64ATUBCDA - Safety Data Sheet
Safety Data Sheet (SPA) a-Tubulin housek Std / 64ATUBCDA
64ATUBCDA - Safety Data Sheet
Safety Data Sheet (ENG-GB) a-Tubulin housek Std / 64ATUBCDA
64ATUBCDA - Safety Data Sheet
Safety Data Sheet (ENG-US) a-Tubulin housek Std / 64ATUBCDA
64ATUBCDA - Safety Data Sheet
Batch Information a-Tubulin housek Std / 20231120
64ATUBCDA Batch 01A - Batch Information
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
Let's find your reader
You might be interested in