Alpha-Tubulin Housekeeping standard

This standard is a spare reagent for the Alpha-Tubulin Housekeeping kit. It may be used for absolute quantification of cellular alpha-tubulin extracted with Cisbio’s lysis buffer.

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  • Developed and tested to Cisbio's high quality standards Developed and tested to Cisbio's high quality standards

This standard is a spare reagent for the Alpha-Tubulin Housekeeping kit. It may be used for absolute quantification of cellular alpha-tubulin extracted with Cisbio’s lysis buffer.

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Overview

This standard provides a way to quantify the alpha tubulin contained in your sample

Benefits

  • PRODUCED BY MANUFACTURER
  • STRINGENT QUALITY CONTROL

Correlation with the BCA protein assay

Lysates of human and mouse cell lines were plated at different cell densities in 96-well culture plates for 5 hours at 37°C, 5% CO2. Cells were then lysed with 50 µL of supplemented lysis buffer #4 for 30 minutes at RT under gentle shaking, and 4 µL of cell lysate were transferred into a low volume white microplate. For HTRF detection, 12 µL of kit diluent were added followed by the dispensing of 4 µL of premixed detection  antibodies. The HTRF signal was recorded after an overnight incubation at RT. In parallel, the concentration of proteins was determined in the same lysates using the BCA protein assay (QuantiPro™ BCA Assay Kit, Sigma-Aldrich) according to the manufacturer’s instructions. For both methods (HTRF or BCA), lysates were analyzed either neat or prediluted in the appropriate buffer in order to work within the linear range of each kit. The cell densities used for the correlations are mentioned on each graph next to each point.

Image of the correlation between HTRF Alpha-Tubulin Housekeeping assay and the BCA protein assay on Hela cells
Image of the correlation between HTRF Alpha-Tubulin Housekeeping assay and the BCA protein assay on NIH3T3 cells
Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Best practices for analyzing brain samples with HTRF® phospho assays for neurosciences

Insider Tips for successful sample treatment - Technical Notes

Optimize your HTRF cell signaling assays on tissues

HTRF and WB compatible guidelines - Technical Notes

Best practices for analyzing tumor xenografts with HTRF phospho assays

Protocol for tumor xenograft analysis with HTRF - Technical Notes

Key guidelines to successful cell signaling experiments

Mastering the art of cell signaling assays optimization - Guides

HTRF® cell signaling platform combined with iCell® Hepatocytes

A solution for phospho-protein analysis in metabolic disorders - Posters

HTRF phospho-assays reveal subtle drug-induced effects

Detailed protocol and direct comparison with WB - Posters

Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells

Analysis of a large panel of diverse biological samples and cellular models - Posters

HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts

Tumor xenograft analysis: HTRF versus Western blot - Application Notes

HTRF cell-based phospho-protein data normalization

Valuable guidelines for efficiently analyzing and interpreting results - Application Notes

HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers

Increased flexibility of phospho-assays - Application Notes

HTRF Alpha-tubulin Housekeeping kit

Properly interpret your compound effect - Application Notes

Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling

Analyse of PI3K/AKT/mTor translational control pathway - Application Notes

Side-by-side comparison of HTRF, Western Blot, ELISA and AlphaScreen® SureFire®

Do all cell-based kinase assays perform similarly? - Posters

Unleash the potential of your phosphorylation research with HTRF

A fun video introducing you to phosphorylation assays with HTRF - Videos

How to run a cell based phospho HTRF assay

3' video to set up your Phospho assay - Videos

How to run a cell based phospho HTRF assay

What to expect at the bench - Videos

HTRF cellular phospho-protein assays

Physiologically relevant results fo fast flowing research - Flyers

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