Human MBNL1 kit
Human MBNL1 quantification in cell lysates
Alpha-Tubulin kit
This HTRF kit is designed for the rapid detection of total alpha tubulin in cell lysates.
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No-wash
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Low sample consumption
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High sensitivity
Overview
Alpha-Tubulin is a member of the tubulin protein superfamily, which polymerizes with the beta tubulin to form microtubules, a major component of the eukaryotic cytoskeleton. Microtubules contribute to several cellular processes, such as structural support, intracellular transport, and DNA segregation. The Alpha-Tubulin kit is designed for the quantitative detection of total Alpha-Tubulin in cell lysates. This assay can be used as a normalization readout.
Benefits
Assay principle
Alpha-Tubulin is measured using a sandwich immunoassay involving two specific anti-alpha-Tubulin antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of the total alpha-Tubulin present in the sample.
Assay Protocol
The protocol for the alpha-Tubulin assay is described here. Cells are plated, stimulated, and lysed in the same 96-well culture plate. Lysates are then transferred to the assay plate for the detection of Alpha-Tubulin by HTRF reagents. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
Product Insert a-Tubulin Kit / 63ADK073PEG-63ADK073PEH
63ADK073PEG-63ADK073PEH - Product Insert
Cisbio Product Catalog 2019
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
Safety Data Sheet (DEU-) a-Tubulin Kit / 63ADK073PEH
63ADK073PEH - Safety Data Sheet
Safety Data Sheet (ELL-) a-Tubulin Kit / 63ADK073PEH
63ADK073PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) a-Tubulin Kit / 63ADK073PEH
63ADK073PEH - Safety Data Sheet
Safety Data Sheet (ITA-) a-Tubulin Kit / 63ADK073PEH
63ADK073PEH - Safety Data Sheet
Safety Data Sheet (SPA-) a-Tubulin Kit / 63ADK073PEH
63ADK073PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) a-Tubulin Kit / 63ADK073PEH
63ADK073PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) a-Tubulin Kit / 63ADK073PEH
63ADK073PEH - Safety Data Sheet