Alpha-Tubulin kit HTRF®

Associated products

This HTRF kit is designed for the rapid detection of total alpha tubulin in cell lysates.

No-wash
Low sample consumption
High sensitivity

Download the protocols

This HTRF kit is designed for the rapid detection of total alpha tubulin in cell lysates.

Size

Cat. number selected :

Quantity

Overview

Alpha-Tubulin is a member of the tubulin protein superfamily, which polymerizes with beta tubulin to form microtubules, a major component of the eukaryotic cytoskeleton. Microtubules contribute to several cellular processes, such as structural support, intracellular transport, and DNA segregation. The Alpha-Tubulin kit is designed for the quantitative detection of total Alpha-Tubulin in cell lysates. This assay can be used as a normalization readout.

Benefits

READOUT NORMALIZATION

Assay principle

Alpha-Tubulin is measured using a sandwich immunoassay involving two specific anti-alpha-Tubulin antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of the total alpha-Tubulin present in the sample.

Assay Protocol

The protocol for the Alpha-Tubulin assay is described here. Cells are plated, stimulated, and lysed in the same 96-well culture plate. Lysates are then transferred into the assay plate for the detection of alpha-Tubulin by HTRF reagents. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.

alpha Tubulin 2-plate assay protocol

12,500 NIH/3T3 cells were plated in 96-well plate and treated after incubation overnight for 16h with compounds at 37°C, CO2. Lysis buffer was added after supernatant removal. 30 minutes under shaking conditions permit the lysate generation and then we transferred the lysatesd to 384-well plate for detection. Trichostatin A (TSA), a potent inhibitor of histone deacetylase, and Tubacin, a selective inhibitor of HDAC6, increase the level of acetylated α –Tubulin on Lysine 40 on NIH/3T3 cells while the expression level of α –Tubulin in presence of both inhibitors remains constant.

Product Insert a-Tubulin Kit / 63ADK073PEG-63ADK073PEH

63ADK073PEG-63ADK073PEH - Product Insert

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Safety Data Sheet (DEU) a-Tubulin Kit / 63ADK073PEG

63ADK073PEG - Safety Data Sheet

Safety Data Sheet (ELL) a-Tubulin Kit / 63ADK073PEG

63ADK073PEG - Safety Data Sheet

Safety Data Sheet (FRA-FR) a-Tubulin Kit / 63ADK073PEG

63ADK073PEG - Safety Data Sheet

Safety Data Sheet (ITA) a-Tubulin Kit / 63ADK073PEG

63ADK073PEG - Safety Data Sheet

Safety Data Sheet (SPA) a-Tubulin Kit / 63ADK073PEG

63ADK073PEG - Safety Data Sheet

Safety Data Sheet (ENG-GB) a-Tubulin Kit / 63ADK073PEG

63ADK073PEG - Safety Data Sheet

Safety Data Sheet (ENG-US) a-Tubulin Kit / 63ADK073PEG

63ADK073PEG - Safety Data Sheet

Safety Data Sheet (DEU-) a-Tubulin Kit / 63ADK073PEH