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Angiotensin AT2R expressing cells labeled with Terbium HTRF®

  • Diagram of Angiotensin AT2 receptor labeled with Tb (transiently expressing cells)
  • Principle of Angiotensin AT2 receptor binding assay using the Tag-lite protocol
  • Diagram of Angiotensin AT2 receptor labeled with Tb (transiently expressing cells)
  • Principle of Angiotensin AT2 receptor binding assay using the Tag-lite protocol
  • Video to introduce you to innovative Tag-lite technology
Tag-lite cells transiently expressing the Angiotensin AT2 receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
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  • Fully functional cell line Fully functional cell line
  • Transient expression Transient expression
  • Ready for binding Ready for binding
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Tag-lite cells transiently expressing the Angiotensin AT2 receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
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Overview

Angiotensin II receptor type 2 or simply AT2 is a GPCR expressed during fetal development. AT2 is involved in vasodilation, inhibition of cell growth, and possibly natriuresis.

Cells expressing the AT2 receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.

Benefits

  • PRE-LABELED WITH TERBIUM
  • FROZEN CELLS, READY TO BE USED
  • CELLS/LIGAND VALIDATED FOR BINDING

Assay principle

Running your AT2 receptor binding assay using Tag-lite is as easy as it can gets. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Diagram of AT2 receptor binding assay using the Tag-lite protocol

Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Diagram of a saturation binding assay using Tag-lite
Representative data obtained when running a saturation binding assay

Watch this video explaining how to run a saturation binding assay using Tag-lite.

Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Diagram of a competitive binding assay using Tag-lite
Representative data obtained when running a competitive binding assay

Watch this video explaining how to run a competitive binding assay using Tag-lite.

Kd and Ki validation

Examples of data obtained using Angiotensin AT2 labeled cells and their matching fluorescent ligand (L0007RED). AT2 was used as reference ligand. Results may vary from one HTRF® compatible reader to another.

Representative saturation binding and competitive binding graph
Representative competitive binding graph
View product citations for GPCRs C1TT1AT2 on CiteAb

Evaluation of a Tag-lite binding assay for a class B receptor

In collaboration with Boehringer Ingelheim - Scientific Presentations

Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery

In collaboration with Bayer - Scientific Presentations

Tag-lite binding assays

The easy way to approach biology without radioactivity - Flyers

Determination of association (kon) and dissociation (koff) rates constants using the Tag-lite platform

Challenge the limits of binding kinetics studies - Application Notes

Understanding GPCRs is the key to improved DD

In collaboration with GEN - Guides

Tag-lite, the easiest solution for studying GPCR-ligands interactions

The gold standard technology for receptor binding studies - Videos

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Webinar | Association Rate Constant: The Unsung Hero of Drug Discovery Kinetics

Available On-demand - Videos

Product Insert AT2 LabCell / C1TT1AT2

C1TT1AT2 - Product Insert

Easy method for kinetic binding determination

How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes

GPCRs Pharmacology

A comprehensive overview of pharmacology's main ligands - Guides

GPCRs: the pathway to Discovery

Complete solutions for GPCR Drug Discovery - Brochures

Safety Data Sheet AT2 LabCell / C1TT1AT2

C1TT1AT2 - Safety Data Sheet

Safety Data Sheet (DEU) AT2 LabCell / C1TT1AT2

C1TT1AT2 - Safety Data Sheet

Safety Data Sheet (ELL) AT2 LabCell / C1TT1AT2

C1TT1AT2 - Safety Data Sheet

Safety Data Sheet (FRA-FR) AT2 LabCell / C1TT1AT2

C1TT1AT2 - Safety Data Sheet

Safety Data Sheet (ITA) AT2 LabCell / C1TT1AT2

C1TT1AT2 - Safety Data Sheet

Safety Data Sheet (SPA) AT2 LabCell / C1TT1AT2

C1TT1AT2 - Safety Data Sheet

Safety Data Sheet (ENG-GB) AT2 LabCell / C1TT1AT2

C1TT1AT2 - Safety Data Sheet

Safety Data Sheet (ENG-US) AT2 LabCell / C1TT1AT2

C1TT1AT2 - Safety Data Sheet

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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