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APP (BACE1 substrate) HTRF®

APP substrate is used in the case of High enzymatic Km to monitor beta-secretase (BACE1) activity.
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APP substrate is used in the case of High enzymatic Km to monitor beta-secretase (BACE1) activity.
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Overview

Beta-secretase (BACE1) is an enzyme which cleaves amyloid precursor protein (APP) to produce the amyloid ß42 peptide, responsible for insoluble amyloid plaques in Alzheimer's disease (AD).

APP substrate, used with HTRF APP substrate labeled with both biotin and Eu3+ Cryptate, enables the study of High enzymatic Km to monitor beta-secretase (BACE1) activity.

Benefits

  • BACE1 ACTIVITY
  • BATCH-TO-BATCH REPRODUCIBILITY
  • ALLOWS HOMOGENEOUS ASSAY

HTRF PPI your dream assay served on a plate

Sandwiches aren't just for eating - Infographics

Best practices for pharmacological characterization of PPI inhibitors

Easy pharmacological characterization of PPI modulators. - Technical Notes

HTRF assays handle low- to high affinity protein-protein interactions

Deciphering low- and high affinity interactions - Application Notes

Nuclear receptor ligand identification with HTRF

Monitoring nuclear receptor binding with HTRF assays - Application Notes

HTRF addresses large protein-protein interaction complexes

Challenge large complexes with HTRF assays - Application Notes

A brief history of Protein-Protein Interactions

How well do you know PPI? - Infographics

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Virology research solutions using HTRF Protein-Protein Interaction assays

See how peer researchers challenge the viral life cycle with PPI assays - Application Notes

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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