cAMP Gs dynamic kit
Optimal performance when applied to Gs activation or inhibition
cAMP Gi kit HTRF®
This HTRF kit detects changes of cAMP accumulation in response to Gi coupled GPCR activation or inhibition
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Ease-of-use
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High sensitivity
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Accurate pharmacology
Overview
Based on HTRF technology, the kit contains all the reagents needed to quantify cAMP, ensuring that you benefit from a complete tool for your needs.
Cisbio's cAMP kits are best suited for measuring cAMP modulations in response to Gi-activation, leveraging an optimized combination of robustness and sensitivity.
Benefits
- SCREENING OF GI COUPLED GPCR
- LEAD OPTIMIZATION OF GI COMPOUNDS
- STUDY OF GI GPCR RESPONSES
Assay principle
cAMP kits are based on a competitive format involving a specific antibody labeled with d2 (acceptor) and cAMP coupled to cryptate (donor). This enables the direct characterization of all types of compounds acting on Gi-coupled receptors in either adherent or suspension cells. Native cAMP produced by cells competes with cryptate -labeled cAMP for binding to monoclonal anti-cAMP.
Assay protocol
The protocol is simple and straightforward, with just two incubation steps: - Cell stimulation by the target compounds - cAMP detection using HTRF reagents
Product specifications
Designed with signal performance in mind, the kit offers a tailored standard curve ideally suited to the study of Gi coupled GPCRs.
| S/B | IC10 nM | IC50 nM | IC90 nM | |
|---|---|---|---|---|
| cAMP - Gi | >31 | 0.8 | 3.0 | 12 |
Introduction to product validation
The cAMP Gi kit allows enhanced performances for Gi coupled receptor assessment.
The data below shows the pharmacological validation of the Gi kit for the stably expressing delta opioid receptor cell line. Agonists and antagonists were well characterized, showing potency in agreement with the literature.
Validation on agonist dose response
CHO cells stably expressing the delta opioid receptor (DOR) at 6000 cells/well were treated with the SNC-162, a reference agonist, for 45 min.
SNC-162 was well characterized, displaying the right potency in good correlation with the literature.
DOR Agonist SNC-162 | cAMP Gi | cAMP Gs dynamic |
| Optimal Cell density / well | 6000 | 12000 |
| S/B | 4.5 | 2.3 |
| EC50 (nM) | 1.6 | 1.9 |
Validation on antagonist dose response
CHO cells stably expressing the delta opioid receptor (DOR) at 6000 cells/well were treated with the SNC-162 agonist for 45 min at the EC90 value (10 nM), which is the optimal concentration to use for the antagonist mode experiment.
Fitting the serial dilutions of the Naltrindole and the Naloxone antagonists enabled IC50 calculation of the compounds (potency), which was in perfect correlation with the literature.
| cAMP Gi | cAMP Gs dynamic | |||
| DOR Antagonists | Naltrindole | Naloxone | Naltrindole | Naloxone |
| Optimal Cells density / well | 6000 | 6000 | 12000 | 12000 |
| S/B | 4 | 4.2 | 2.3 | 2.3 |
| IC50 (nM) | 1.4 | 868 | 2.4 | 934 |
In collaboration with CNRS - Scientific Presentations
In collaboration with the Faculty of health and medical sciences Denmark - Scientific Presentations
In collaboration with MRC technology - Scientific Presentations
In collaboration with Novartis - Scientific Presentations
IP-One & cAMP HTRF Functional Cell-based Screening
In collaboration with Schering AG - Scientific Presentations
Biomarker and Cell Signaling Assays for Fibrosis and NASH
HTRF and Alpha solutions for NASH - Flyers
HTRF products and supporting scientific content to investifgate GPCRs - Flyers
Application of Bioassay Performance Monitoring Process with Statistical Tools
In collaboration with Amylin Pharmaceuticals Inc., Biocon Limited - Posters
In collaboration with Molecular Devices - Posters
In collaboration with MRC - Posters
Nanobodies and HTRF® technology to address an orphan class B GPCR: The GPR97 receptor
In collaboration with Sanofi - Posters
Characterisation of Inositol Phosphate & cAMP Signalling from GPCRs using HTRF
In collaboration with College of Science, King Saud University, University of Western Australia - Posters
HTRF® technology on the SpectraMax® i3 Multi-Mode Platform
In collaboration with Mollecular Devices - Posters
Structure-activity characterization of glucagon receptor, an example of using HTRF®cAMP assay
In collaboration with Chinese Academy of Sciences - Posters
Webinar: Advancing GPCR drug discovery through Allosteric Modulation
Featuring a panel of experts - Videos
Ultimate guides to master Gs and Gi GPCR assays
Optimizing cAMP assays has never been easier - Guides
Webinar: High-Throughput Screening of GPCRs for Drug Discovery Applications
Featuring a panel of experts - Videos
HTRF and Tag-lite technologies in multidimensional cell-based assays for GPCR investigations
16 GPCR challenges, 1 solution - Posters
Understanding GPCRs is the key to improved DD
In collaboration with GEN - Guides
Guidelines and recommendations for analyzing GPCR 2nd messenger data
Written by a panel of experts - Guides
Identifying therapeutic candidates for Schizophrenia through HTS - Posters
What to expect at the bench - Videos
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Immuno-Oncology Drug Discovery
Explore our solutions for Immuno-oncology research - Flyers
Molecular basis of neuroinflammation and neurodegeneration diseases
The essential guide for extending your knowledge on the molecular mechanisms of neurodegenerative diseases - Guides
Neurodegeneration and its main related diseases
Discover this infographic design on neurodegenerative diseases - Infographics
A comprehensive overview of pharmacology's main ligands - Guides
GTP binding assay : Gαi agonist and antagonist guides
Discover how to get the most out of your Gαi agonist and antagonist research with our HTRF GTP binding assays. - Guides
GPCR compounds identification and pharmacological characterization with GTP Gi binding assay
Characterization of candidate molecules through GTP binding assays - Application Notes
GPCR in Cardiovascular Research: The Relevance of Camp and IP-1 Second Messenger Assays
See how peer researchers monitor GPCR signaling with HTRF - Application Notes
Upstream/Downstream GPCR Readout Monitoring with GTP Gi Binding and cAMP Gi HTRF Assays
Read this note featuring two case studies that demonstrate the relevance of combining upstream (GTP) and downstream (cAMP) readouts for characterizing pharmacological compounds - Application Notes
GPCRs: the pathway to Discovery
Complete solutions for GPCR Drug Discovery - Brochures
Manual cAMP Gi / 62AM9PEB-62AM9PEC-62AM9PEJ
62AM9PEB-62AM9PEC-62AM9PEJ - Product Insert
Certificate of Analysis cAMP Gi / 62AM9PEB
62AM9PEB Batch 06L - Quality Control Report
Certificate of Analysis cAMP Gi / 62AM9PEB
62AM9PEB Batch 06M - Quality Control Report
Certificate of Analysis cAMP Gi / 62AM9PEB
62AM9PEB Batch 06N - Quality Control Report
Certificate of Analysis cAMP Gi / 62AM9PEB
62AM9PEB Batch 06P - Quality Control Report
Safety Data Sheet (DEU) cAMP Gi / 62AM9PEB
62AM9PEB - Safety Data Sheet
Safety Data Sheet (ELL) cAMP Gi / 62AM9PEB
62AM9PEB - Safety Data Sheet
Safety Data Sheet (FRA-FR) cAMP Gi / 62AM9PEB
62AM9PEB - Safety Data Sheet
Safety Data Sheet (ITA) cAMP Gi / 62AM9PEB
62AM9PEB - Safety Data Sheet
Safety Data Sheet (SPA) cAMP Gi / 62AM9PEB
62AM9PEB - Safety Data Sheet
Safety Data Sheet (ENG-GB) cAMP Gi / 62AM9PEB
62AM9PEB - Safety Data Sheet
Safety Data Sheet (ENG-US) cAMP Gi / 62AM9PEB
62AM9PEB - Safety Data Sheet
Certificate of Analysis cAMP Gi / 62AM9PEC
62AM9PEC Batch 06F - Quality Control Report
Certificate of Analysis cAMP Gi / 62AM9PEC
62AM9PEC Batch 07A - Quality Control Report
Safety Data Sheet (DEU) cAMP Gi / 62AM9PEC
62AM9PEC - Safety Data Sheet
Safety Data Sheet (ELL) cAMP Gi / 62AM9PEC
62AM9PEC - Safety Data Sheet
Safety Data Sheet (FRA-FR) cAMP Gi / 62AM9PEC
62AM9PEC - Safety Data Sheet
Safety Data Sheet (ITA) cAMP Gi / 62AM9PEC
62AM9PEC - Safety Data Sheet
Safety Data Sheet (SPA) cAMP Gi / 62AM9PEC
62AM9PEC - Safety Data Sheet
Safety Data Sheet (ENG-GB) cAMP Gi / 62AM9PEC
62AM9PEC - Safety Data Sheet
Safety Data Sheet (ENG-US) cAMP Gi / 62AM9PEC
62AM9PEC - Safety Data Sheet
Certificate of Analysis cAMP Gi / 62AM9PEJ
62AM9PEJ Batch 06F - Quality Control Report
Certificate of Analysis cAMP Gi / 62AM9PEJ
62AM9PEJ Batch 06G - Quality Control Report
Certificate of Analysis cAMP Gi / 62AM9PEJ
62AM9PEJ Batch 07A - Quality Control Report
Certificate of Analysis cAMP Gi / 62AM9PEJ
62AM9PEJ Batch 07B - Quality Control Report
Safety Data Sheet (DEU) cAMP Gi / 62AM9PEJ
62AM9PEJ - Safety Data Sheet
Safety Data Sheet (ELL) cAMP Gi / 62AM9PEJ
62AM9PEJ - Safety Data Sheet
Safety Data Sheet (FRA-FR) cAMP Gi / 62AM9PEJ
62AM9PEJ - Safety Data Sheet
Safety Data Sheet (ITA) cAMP Gi / 62AM9PEJ
62AM9PEJ - Safety Data Sheet
Safety Data Sheet (SPA) cAMP Gi / 62AM9PEJ
62AM9PEJ - Safety Data Sheet
Safety Data Sheet (ENG-GB) cAMP Gi / 62AM9PEJ
62AM9PEJ - Safety Data Sheet
Safety Data Sheet (ENG-US) cAMP Gi / 62AM9PEJ
62AM9PEJ - Safety Data Sheet
Certificate of Analysis cAMP Gi / 64AM8PEJ
64AM8PEJ Batch 14A - Quality Control Report
Safety Data Sheet (DEU) cAMP Gi / 64AM8PEJ
64AM8PEJ - Safety Data Sheet
Safety Data Sheet (ELL) cAMP Gi / 64AM8PEJ
64AM8PEJ - Safety Data Sheet
Safety Data Sheet (FRA-FR) cAMP Gi / 64AM8PEJ
64AM8PEJ - Safety Data Sheet
Safety Data Sheet (ITA) cAMP Gi / 64AM8PEJ
64AM8PEJ - Safety Data Sheet
Safety Data Sheet (SPA) cAMP Gi / 64AM8PEJ
64AM8PEJ - Safety Data Sheet
Safety Data Sheet (ENG-GB) cAMP Gi / 64AM8PEJ
64AM8PEJ - Safety Data Sheet
Safety Data Sheet (ENG-US) cAMP Gi / 64AM8PEJ
64AM8PEJ - Safety Data Sheet
Certificate of Analysis cAMP Gi / 64AM8PEJ
64AM8PEJ Batch 15A - Quality Control Report
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