cAMP Gs dynamic kit
Optimal performance when applied to Gs activation or inhibition
cAMP Gs HiRange kit HTRF®
This HTRF kit detects changes of cAMP accumulation in response to Gs coupled GPCR activation or inhibition
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Ease-of-use
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High sensitivity
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Accurate pharmacology
Overview
Based on HTRF technology, the kit contains all the reagents needed to quantify cAMP, ensuring that you benefit from a complete tool for your needs.
Cisbio's cAMP kits are best suited for measuring cAMP modulations in response to Gs-activation, leveraging an optimized combination of robustness and sensitivity
Benefits
- SCREENING OF GS COUPLED GPCR
- LEAD OPTIMIZATION OF GS COMPOUNDS
- STUDY OF GS GPCR RESPONSES
Assay principle
Cisbio cAMP kits are based on a competitive format involving a specific antibody labeled with cryptate (donor) and cAMP coupled to d2 (acceptor). This enables the direct characterization of all types of compounds acting on Gs-coupled receptors in either adherent or suspension cells.
Assay protocol
The protocol is simple and straightforward, with just two incubation steps: - Cell stimulation by the target compounds - cAMP detection using HTRF reagents
cAMP Gs hirange product specifications
If your experimental design uses membrane preparations, lipoparticles or purified receptors, or if your samples have large quantities of cAMP, the Gs HiRange kit will fit your needs!
The kit shows no ATP cross-reactivity up to 10mM, allowing the user to operate with large ranges of ATP and enabling membrane work.
Designed for optimal signal performance, cAMP - Gs HiRange offers a maximized signal-to-background score.
| S/B | IC20 nM | IC50 nM | IC80 nM | |
|---|---|---|---|---|
| cAMP - Gs dynamic | >14 | 0.91 | 4.07 | 18.1 |
| cAMP - Gs HiRange | >28 | 4.27 | 22.8 | 121 |
ATP cross-reactivity
In collaboration with CNRS - Scientific Presentations
In collaboration with the Faculty of health and medical sciences Denmark - Scientific Presentations
In collaboration with the Faculty of health and medical sciences Denmark - Scientific Presentations
In collaboration with MRC technology - Scientific Presentations
HTRF a versatil approach for 7TM drug discovery
In collaboration with MRC - Scientific Presentations
In collaboration with Novartis - Scientific Presentations
cAMP Fresh and Frozen Cell Usage
In collaboration with Bristol Myers Squibb - Scientific Presentations
IP-One & cAMP HTRF Functional Cell-based Screening
In collaboration with Schering AG - Scientific Presentations
Biomarker and Cell Signaling Assays for Fibrosis and NASH
HTRF and Alpha solutions for NASH - Flyers
HTRF products and supporting scientific content to investifgate GPCRs - Flyers
Application of Bioassay Performance Monitoring Process with Statistical Tools
In collaboration with Amylin Pharmaceuticals Inc., Biocon Limited - Posters
In collaboration with Molecular Devices - Posters
Nanobodies and HTRF® technology to address an orphan class B GPCR: The GPR97 receptor
In collaboration with Sanofi - Posters
Characterisation of Inositol Phosphate & cAMP Signalling from GPCRs using HTRF
In collaboration with College of Science, King Saud University, University of Western Australia - Posters
HTRF® technology on the SpectraMax® i3 Multi-Mode Platform
In collaboration with Mollecular Devices - Posters
Structure-activity characterization of glucagon receptor, an example of using HTRF®cAMP assay
In collaboration with Chinese Academy of Sciences - Posters
Multidimentional GPCR investigations - Posters
Webinar: Advancing GPCR drug discovery through Allosteric Modulation
Featuring a panel of experts - Videos
Kinetics of the ß2 Adrenergic receptor binding and signaling
A look at the kinetics of cAMP production in cells - Application Notes
Ultimate guides to master Gs and Gi GPCR assays
Optimizing cAMP assays has never been easier - Guides
Enhanced performance of the new HTRF® cAMP kit on Gai/o coupled GPCR
Kit specifically designed to investigate Gai/o signaling - Posters
Webinar: High-Throughput Screening of GPCRs for Drug Discovery Applications
Featuring a panel of experts - Videos
HTRF and Tag-lite technologies in multidimensional cell-based assays for GPCR investigations
16 GPCR challenges, 1 solution - Posters
Understanding GPCRs is the key to improved DD
In collaboration with GEN - Guides
Guidelines and recommendations for analyzing GPCR 2nd messenger data
Written by a panel of experts - Guides
What to expect at the bench - Videos
Product Insert cAMP Gs HiRg / 62AM6PEB-62AM6PEC-62AM6PEJ
62AM6PEB-62AM6PEC-62AM6PEJ - Product Insert
Quantitate cAMP and TNF Utilizing the Synergy 2 Multi-Detection Microplate Reader and HTRF assays
BioTek - Application Notes
HTRF cAMP HiRange Assay on SpectraMax Multi-Mode Microplate Readers
Molecular Devices - Application Notes
HTRF technology on the SpectraMax i3 Multi‐Mode Platform
Molecular Devices - Posters
Homogeneous High Throughput Live Cell GPCR Functional and Surface Binding Assays
Molecular Devices - Posters
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
GPCR in Cardiovascular Research: The Relevance of Camp and IP-1 Second Messenger Assays
See how peer researchers monitor GPCR signaling with HTRF - Application Notes
Molecular basis of neuroinflammation and neurodegeneration diseases
The essential guide for extending your knowledge on the molecular mechanisms of neurodegenerative diseases - Guides
Neurodegeneration and its main related diseases
Discover this infographic design on neurodegenerative diseases - Infographics
A comprehensive overview of pharmacology's main ligands - Guides
GPCR compounds identification and pharmacological characterization with GTP Gi binding assay
Characterization of candidate molecules through GTP binding assays - Application Notes
Upstream/Downstream GPCR Readout Monitoring with GTP Gi Binding and cAMP Gi HTRF Assays
Read this note featuring two case studies that demonstrate the relevance of combining upstream (GTP) and downstream (cAMP) readouts for characterizing pharmacological compounds - Application Notes
GPCRs: the pathway to Discovery
Complete solutions for GPCR Drug Discovery - Brochures
Safety Data Sheet cAMP Gs HiRg / 62AM6PEB
62AM6PEB - Safety Data Sheet
Safety Data Sheet (DEU) cAMP Gs HiRg / 62AM6PEB
62AM6PEB - Safety Data Sheet
Safety Data Sheet (ELL) cAMP Gs HiRg / 62AM6PEB
62AM6PEB - Safety Data Sheet
Safety Data Sheet (FRA-FR) cAMP Gs HiRg / 62AM6PEB
62AM6PEB - Safety Data Sheet
Safety Data Sheet (ITA) cAMP Gs HiRg / 62AM6PEB
62AM6PEB - Safety Data Sheet
Safety Data Sheet (SPA) cAMP Gs HiRg / 62AM6PEB
62AM6PEB - Safety Data Sheet
Safety Data Sheet (ENG-GB) cAMP Gs HiRg / 62AM6PEB
62AM6PEB - Safety Data Sheet
Safety Data Sheet (ENG-US) cAMP Gs HiRg / 62AM6PEB
62AM6PEB - Safety Data Sheet
Safety Data Sheet cAMP Gs HiRg / 62AM6PEC
62AM6PEC - Safety Data Sheet
Safety Data Sheet (DEU) cAMP Gs HiRg / 62AM6PEC
62AM6PEC - Safety Data Sheet
Safety Data Sheet (ELL) cAMP Gs HiRg / 62AM6PEC
62AM6PEC - Safety Data Sheet
Safety Data Sheet (FRA-FR) cAMP Gs HiRg / 62AM6PEC
62AM6PEC - Safety Data Sheet
Safety Data Sheet (ITA) cAMP Gs HiRg / 62AM6PEC
62AM6PEC - Safety Data Sheet
Safety Data Sheet (SPA) cAMP Gs HiRg / 62AM6PEC
62AM6PEC - Safety Data Sheet
Safety Data Sheet (ENG-GB) cAMP Gs HiRg / 62AM6PEC
62AM6PEC - Safety Data Sheet
Safety Data Sheet (ENG-US) cAMP Gs HiRg / 62AM6PEC
62AM6PEC - Safety Data Sheet
Safety Data Sheet (DEU) cAMP Gs HiRg / 62AM10PEC
62AM10PEC - Safety Data Sheet
Safety Data Sheet (ELL) cAMP Gs HiRg / 62AM10PEC
62AM10PEC - Safety Data Sheet
Safety Data Sheet (FRA-FR) cAMP Gs HiRg / 62AM10PEC
62AM10PEC - Safety Data Sheet
Safety Data Sheet (ITA) cAMP Gs HiRg / 62AM10PEC
62AM10PEC - Safety Data Sheet
Safety Data Sheet (SPA) cAMP Gs HiRg / 62AM10PEC
62AM10PEC - Safety Data Sheet
Safety Data Sheet (ENG-GB) cAMP Gs HiRg / 62AM10PEC
62AM10PEC - Safety Data Sheet
Safety Data Sheet (ENG-US) cAMP Gs HiRg / 62AM10PEC
62AM10PEC - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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