CD16a (FcgRIIIa, V158) cellular binding kit
CD16a (FcgRIIIa, V158) cellular binding assay for ADCC prediction
CD32a (FcgRIIa, H131) cellular binding kit HTRF®
The CD32a cellular binding kit is designed for monitoring the binding of the IgG Fc region to the human CD32a receptor on live cells.
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Cellular binding assay
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No-wash
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All inclusive kit
Overview
The CD32a (FcgRIIa, H131) cellular binding assay is a simple and robust test to monitor binding of the IgG Fc region to the human CD32a receptorin live cells and to select the most potent antibody for use in Antibody-Dependent Cell-Phagocytosis (ADCP). Two separate CD32a assays are available to specifically target the H131 or R131 variants. These assays, based on our innovative Tag-lite technology, can easily be automated to create a high-throughput solution for predicting ADCP.
Benefits
- FAST ANTIBODY SELECTION
- ROBUSTNESS
- LOW SAMPLE CONSUMPTION
Assay principle
The CD32a receptor cellular binding assay is a competitive assay involving HEK293 cells expressing a specific CD32a variant conjugated to Lumi4-terbium Cryptate, and human IgG1 labelled with d2. The unlabelled antibody or Fc tagged drug competes with the d2 labelled IgG1 for binding to the receptor, resulting in a decrease of FRET signal.
Assay protocol
The CD32a receptor cellular binding assays (FcgIIa H131 or R131) present only 1 incubation step after reagents distribution. Lumi4-terbium Cryptate pre-labelled cells are dispensed, followed by the addition of IgG1 labelled with d2 and the sample. After 2h incubation at RT, the signal can be read.
Tag-lite® services to evaluate your Biosimilar or Biobetter samples
profile and study your Fcg receptor - Flyers
A cellular binding platform to measure human IgG affinity for Fc gamma receptors (FcyRs)
Characterizing therapeutic antibodies - Posters
Therapeutic development with robust cellular binding assays
Tag-lite® CD16a cellular binding assays - Flyers
Manual CD32a (H131) Kit / 6FRAHPAG-6FRAHPAE-6FRAHPAF
6FRAHPAG-6FRAHPAE-6FRAHPAF - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Safety Data Sheet (DEU) CD32a (H131) Kit / 6FRAHPAE
6FRAHPAE - Safety Data Sheet
Safety Data Sheet (ELL) CD32a (H131) Kit / 6FRAHPAE
6FRAHPAE - Safety Data Sheet
Safety Data Sheet (FRA-FR) CD32a (H131) Kit / 6FRAHPAE
6FRAHPAE - Safety Data Sheet
Safety Data Sheet (ITA) CD32a (H131) Kit / 6FRAHPAE
6FRAHPAE - Safety Data Sheet
Safety Data Sheet (SPA) CD32a (H131) Kit / 6FRAHPAE
6FRAHPAE - Safety Data Sheet
Safety Data Sheet (ENG-GB) CD32a (H131) Kit / 6FRAHPAE
6FRAHPAE - Safety Data Sheet
Safety Data Sheet (ENG-US) CD32a (H131) Kit / 6FRAHPAE
6FRAHPAE - Safety Data Sheet
Safety Data Sheet (DEU) CD32a (H131) Kit / 6FRAHPAF
6FRAHPAF - Safety Data Sheet
Safety Data Sheet (ELL) CD32a (H131) Kit / 6FRAHPAF
6FRAHPAF - Safety Data Sheet
Safety Data Sheet (FRA-FR) CD32a (H131) Kit / 6FRAHPAF
6FRAHPAF - Safety Data Sheet
Safety Data Sheet (ITA) CD32a (H131) Kit / 6FRAHPAF
6FRAHPAF - Safety Data Sheet
Safety Data Sheet (SPA) CD32a (H131) Kit / 6FRAHPAF
6FRAHPAF - Safety Data Sheet
Safety Data Sheet (ENG-GB) CD32a (H131) Kit / 6FRAHPAF
6FRAHPAF - Safety Data Sheet
Safety Data Sheet (ENG-US) CD32a (H131) Kit / 6FRAHPAF
6FRAHPAF - Safety Data Sheet
Safety Data Sheet (DEU) CD32a (H131) Kit / 6FRAHPAG
6FRAHPAG - Safety Data Sheet
Safety Data Sheet (ELL) CD32a (H131) Kit / 6FRAHPAG
6FRAHPAG - Safety Data Sheet
Safety Data Sheet (FRA-FR) CD32a (H131) Kit / 6FRAHPAG
6FRAHPAG - Safety Data Sheet
Safety Data Sheet (ITA) CD32a (H131) Kit / 6FRAHPAG
6FRAHPAG - Safety Data Sheet
Safety Data Sheet (SPA) CD32a (H131) Kit / 6FRAHPAG
6FRAHPAG - Safety Data Sheet
Safety Data Sheet (ENG-GB) CD32a (H131) Kit / 6FRAHPAG
6FRAHPAG - Safety Data Sheet
Safety Data Sheet (ENG-US) CD32a (H131) Kit / 6FRAHPAG
6FRAHPAG - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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