The CD32b cellular binding kit is designed for monitoring the binding of the IgG Fc region to the human CD32b receptor on live cells.
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  • Cellular binding assay
  • No-wash
  • All inclusive kit
The CD32b cellular binding kit is designed for monitoring the binding of the IgG Fc region to the human CD32b receptor on live cells.
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In stock

Overview

The CD32b (FcgRIIb) cellular binding assay is a simple and robust test to monitor binding of the IgG Fc region to the human CD32b receptor in live cells. The CD32b receptor negatively modulates the Antibody-Dependent Cell-Phagocytosis (ADCP) pathway. This assay, based on our innovative Tag-lite technology, can easily be automated to create a high-throughput solution for predicting ADCP.

Benefits

  • FAST ANTIBODY SELECTION
  • ROBUSTNESS
  • LOW SAMPLE CONSUMPTION

Assay principle

The CD32b receptor cellular binding assay is a competitive assay involving HEK293 cells expressing a specific CD32b variant conjugated to Lumi4-terbium Cryptate, and human IgG1 labelled with d2. The unlabelled antibody or Fc tagged drug competes with the d2 labelled IgG1 for binding to the receptor, resulting in a decrease of FRET signal.

Assay protocol

The CD32b receptor cellular binding assays (FcgIIb) present only 1 incubation step after reagents distribution. Lumi4-terbium Cryptate pre-labelled cells are dispensed, followed by the addition of IgG1 labelled with d2 and the sample. After 2h incubation at RT, the signal can be read.
Product Insert CD32b Kit / 6FR2BPAG-6FR2BPAE-6FR2BPAF

6FR2BPAG-6FR2BPAE-6FR2BPAF - Product Insert

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