J. Vallaghé1, D. Monchiet1, N. Costy1, M. Linnebacher2, T. Chardès3, C. Larbouret3, J. Pannequin4, S. Dalle4, E. Trinquet1, F. Charrier-Savournin1
1Cisbio Bioassays, Codolet, France | 2University of Rostock, Germany | 3Institut de Recherche en Cancérologie de Montpellier, INSERM U896, Université Montpellier 1, Institut régional du Cancer Montpellier / Val d’Aurelle, France | 4Institut de Génomique F
High-throughput screening (HTS) on two-dimensional (2D) immortalized cell cultures is frequently the starting point for identifying promising new drugs. To easily and rapidly translate drug discovery research from simplistic in vitro to physiologically relevant in vivo models, researchers need robust biochemical assays compatible with samples presenting different levels of complexity.
This study demonstrates that the HTRF phospho-/total protein platform is suitable for the rapid and robust analysis of cell signaling pathways in different kinds of biological models such as 2D, 3D cell cultures, patient-derived primary cells, and xenografts.