The CHO Host Cell Protein kit is designed for the quantitative assessment of CHO cells contaminants in bioprocess operations.
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The CHO Host Cell Protein kit is designed for the quantitative assessment of CHO cells contaminants in bioprocess operations.
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Overview

The CHO Host Cell Proteins (HCP) kit measures contaminants originating from the CHO cells used to manufacture therapeutic antibodies. The kit can be used for the quantification of CHO HCP proteins in routine bioprocess operations, from the very crude harvest material to the final product. The simple and robust procedure benefits from increased throughput compared to ELISA.

Assay principle

The CHO Host Cell Protein (HCP) assay is a sandwich immunoassay. The assay uses two specific polyclonal antibodies, one labelled with Eu3+-Cryptate and the other with d2. The specific signal is proportional to the concentration of CHO HCP present in the sample. This homogeneous assay requires only a single reagent addition (by premixing detecting reagents) and 2 hours to ON incubation for the quantitative detection of CHO HCP.

Assay protocol

The CHO HCP assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. This protocol requires only 1 single, ON incubation period.

Specifications

Detection Limit: 2 ng/mL
Dynamic Range: 2 to 100 ng/mL
Incubation Time: ON at RT (2H with lower sensitivity)

Specifications

HTRF technology allows multiple readout using the same plate. Figure 1 describes CHO HCP calibration curve in 20µL final using a white 384 well small volume plate. Readout on PHERAstar FS (Laser) was performed after 2h and ON incubation period.
Accelerate throughput without compromising on performance

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Cisbio Product Catalog 2019

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