AlphaLISA Assay Principle
AlphaLISA is a bead-based, no-wash luminescent technology. When Alpha Donor and Acceptor beads are brought together through binding to the target, a cascade of chemical reactions is set in motion, creating an amplified signal. These versatile beads are conjugated with various biomolecules that enable the detection of unique biological events, offering maximum flexibility.
How AlphaLISA technology assays work
An AlphaLISA assay is easy to run, and requires few steps:
First add your analyte or sample to your microplate, then add acceptor beads and antibodies. Incubate 1 hour. Add donor beads and incubate 30-60 minutes. The interaction between bead-bound molecules and analyte brings the bead into proximity. Excite the donor beads with red light. A singlet oxygen is generated by the donor beads, which excites the acceptor beads and will emit light proportional to the level of interaction.
How AlphaLISA advances your science
Ease of use with no wash and no separation steps, fast results in 3 hours for most applications, high sensitivity to detect down to femtogram levels of targets, large dynamic range up to 4-5 logs, and a compatibility with many sample types definitely make Alpha an optimal choice when it comes to moving your research a step forward!
No washing required
Broad sample compatibility
Small sample size
Wide detectable range
Choose the Alpha solution that’s right for you
When running large numbers of ELISA assays from the same sample or working with a difficult matrix, such as serum, or plasma
AlphaLISA® SureFire® Ultra™
For studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors,
Perfect for studying fusion-tagged proteins or protein-protein interactions (PPIs)
When your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own assay
AlphaLISA® SureFire® Ultra™ Multiplex
When looking to measure both phosphorylated and total proteins in the same sample
A novel method for measuring target engagement in cells
Alpha technology expanding research possibilities
Greater confidence in your results with powerful microplate readers
PerkinElmer’s portfolio of plate readers provides the latest detection technologies to meet the diverse assay requirements of labs today. VICTOR® Nivo™, EnSight® or EnVision® can all be equipped for Alpha detection.
Industry’s Smallest Multimode Plate Reader
Unique combination of industry’s leading technologies
Our fastest benchtop multimode plate reader