Home
HTRF small molecule labeling

HTRF small molecule labeling

We label many types of materials!

We label small molecules, nucleic acids…

In the field of drug discovery, researchers are constantly seeking small molecules with therapeutic applications.

Every scientist knows that small molecule labeling often requires a highly specific chemical approach and purification process.

The chemistry team at PerkinElmer | Cisbio has unmatched experience in small molecule bioconjugation.

Solid experience with custom labeling

Our offering includes:

Labeling of small molecules with Europium & Terbium cryptates:
- Aromatics & heterocycle compounds
- Steroids
- Saccharides
- Nucleosides
Derivatization of pharmacophore
Introduction of linkers prior to labeling, to retain activity
Reproduction of a synthesis described in the literature

A solid experience on custom labeling techniques

PerkinElmer | Cisbio excels in services. Our expertise is backed by our comprehensive understanding of chemistry and a commitment to R&D investment.

Pharmaceutical companies, biotechs, and academic laboratories looking to outsource their small molecule labeling projects often find us to be the ideal partner. Let’s explore why.

Our chemists are our greatest asset

Scientists in the PerkinElmer | Cisbio Chemistry Laboratories have unique researcher-developer profiles, a double competence from which your project is now able to benefit from in many ways. Selecting us for your small molecule work is the promise of bringing in a team with unmatched expertise in chemistry.

Highly trained chemists (at PhD., Master’s, and Technician levels) with 10 to 20 years of experience
Access to scientists that are able to combine their understanding of the research approach with the reality of industry
Experience gained from both internal and external projects, through a large collaboration network in academia and industry
Experience gained from long-standing relationships with key opinion leaders in chemistry such as J. M. Lehn (Nobel Prize), David Parker (Durham University), and Marcel Hibert (Strasbourg University)
Your material receives the same attention and discerning synthesis techniques as the material PerkinElmer | Cisbio uses for its own products

Our publications, our credibility

PerkinElmer | Cisbio’s chemists devote their time to synthetizing different molecules, in particular the fluorophores vital to our offering, and managing clients’ custom projects. The remainder of their time is spent on pure chemistry research, advancing their knowledge and developing new processes and techniques, thus pushing the boundaries of the field of chemistry. Below is a selection of their work:

The Journal of Organic Chemistry

Arán, V.J., Kumar, M., Molina, J., Lamarque, L., Navarro, P., García-España, E., Ramirez, J.A., Luis, S.V. and Escuder, B., 1999. Synthesis and protonation behavior of 26-membered oxaaza and polyaza macrocycles containing two heteroaromatic units of 3, 5-disubstituted pyrazole or 1-benzylpyrazole. A potentiometric and 1H and 13C NMR study. The Journal of Organic Chemistry, 64(17), pp.6135-6146.

Chemical Communications

Walton, J.W., Bourdolle, A., Butler, S.J., Soulie, M., Delbianco, M., McMahon, B.K., Pal, R., Puschmann, H., Zwier, J.M., Lamarque, L. and Maury, O., 2013. Very bright europium complexes that stain cellular mitochondria. Chemical Communications, 49(16), pp.1600-1602.

The Journal of American Chemical Society

Miranda, C., Escartí, F., Lamarque, L., Yunta, M.J., Navarro, P., García-España, E. and Jimeno, M.L., 2004. New 1 H-pyrazole-containing polyamine receptors able to complex l-glutamate in water at physiological pH values. Journal of the American Chemical Society, 126(3), pp.823-833.

Chemical Sciences

Butler, S.J., Lamarque, L., Pal, R. and Parker, D., 2014. EuroTracker dyes: highly emissive europium complexes as alternative organelle stains for live cell imaging. Chemical Science, 5(5), pp.1750-1756.Nature Chemical Biology Albizu, L., Cottet, M., Kralikova, M., Stoev, S., Seyer, R., Brabet, I., Roux, T., Bazin, H., Bourrier, E., Lamarque, L. and Breton, C., 2010. Time-resolved FRET between GPCR ligands reveals oligomers in native tissues. Nature chemical biology, 6(8), p.587.

Inorganic chemistry

Bourdolle, Adrien, et al. “Modulating the photophysical properties of azamacrocyclic europium complexes with charge-transfer antenna chromophores.” Inorganic chemistry50.11 (2011): 4987-4999

Our molecules speak for our expertise

The synthesis of a europium tris-bipyridine cryptate (Eu(K)) is no walk in the park to say the least. Europium Cryptate is one of the complex molecules that PerkinElmer | Cisbio chemists synthesize on a monthly basis to supply our assay development scientists with enough material to create new assays and reagents.

Eu(K) is a testament to the skills of our chemists.

Flexibility and customer support

At PerkinElmer | Cisbio, we understand that there is no single solution for the synthesis of your ideal molecule, and that every project brings its own set of challenges. We also understand that every project is unique and will not fit a standard template.

With PerkinElmer | Cisbio, you will receive a complimentary proposal and customized approach to your project from a dedicated chemist.

A clear and straightforward process

Small molecule labeling process

Deliverables include:

To supply a compound with high purity level
To package exact quantities based on UV quantification
To determine a process to solubilize the conjugated molecule
To supply safety documents (MSDS files)
To supply an Analysis certificate including HPLC / UPLC traces, UV and MS spectra.
To supply HRMS analysis on request
Access to NMR facilities to confirm structures (if scale and type of molecule are compatible with such analysis)
Vial label with solvent- and temperature-resistant inks

Next-generation facilities

The chemistry labs are located in France, and are equipped with the latest technology. PerkinElmer | Cisbio’s labs and scientists have the latest equipment, ready to deliver the best possible synthetic chemistry service and the most precise analytical reporting.

Equipment includes:

Several fume hoods
Rotating evaporators under fume hoods, ensuring a healthy laboratory atmosphere
UPLC MS for reaction monitoring
Semi preparative HPLC (purification of small quantities)
Preparative HPLC (up to 100 mg)
Automatized chromatography purification (Reveleris)
Large set of HPLC columns
Speed vac for solvent removal
Lyophilizer
Microwave reactor (CEM) to speed up reactions and reduce side reactions
Mass spectrometer (simple quadripole) coupled to UPLC in a safe room away from solvent
Large number of chemical reagents stored in safe room site
Reaction under inert atmosphere (argon line)
Reaction at low temperature
Hydrogenation reactor facilities
Reaction under pressure with steel reactor
UV spectrometer

I label your small molecules to make them shine brightly in your research!

Stéphanie

I create fluorescent dyes to unleash your assays’ performance!

Laurent

I help create the chemical tools you need to stay focused on your assays.

Emmanuel

Meet the whole custom solution team

Take the tour!

Explore our US and Europe labs to check our latest equipment, and look around our chemistry lab where small molecule labeling magic occurs!

Visit our labs

Ready to discuss your project?

Cisbio’s knowledgeable team of scientists are ready to help you.
No matter what the inquiry, our commitment to exceptional service ensures that we will provide you an optimal solution.