Aldosterone kit
Aldosterone for accurate assessment in supernatant and serum
Cortisol kit HTRF®
The Cortisol kit is intended to quantify hydrocortisone from cells, serum, and liver microsomes.
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High sensitivity
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Highly specific
Overview
Cortisol, also known as hydrocortisone, is the major glucocorticoid produced and secreted by the adrenal cortex. It is involved in regulation of glucose metabolism, fat degradation, blood pressure levels, and stress inhibition. Cortisol also diffuses into cells, where its level is highly regulated by 11ß-hydroxysteroid dehydrogenase (type 1 or 2). The cortisol assay allows for rapid and accurate cortisol measurement, even in complex samples such as liver microsomes, whole cells, and animal serum. This assay can also be used to assess 11ß-HSD1 activity (native, recombinant, or microsomal).
Benefits
- LOW SAMPLE CONSUMPTION
- VALIDATED FOR 11ß-HSD1 MONITORING
Assay principle
The kit is based on a competitive format involving a specific antibody labelled with Cryptate (donor) and cortisol coupled to d2 (acceptor). Cortisol produced by cells competes with d2-labelled cortisol for binding to monoclonal anti-cortisol Eu Cryptate.
Assay protocol
The cortisol assay features a streamlined protocol with only two incubation steps:
- Cell stimulation by target compounds
- Cortisol detection using HTRF reagents.
This protocol requires only a single, two-hour incubation period following cell stimulation.
Key features
| Sample compatibility: | serum, whole cells, &liver microsomes |
|---|---|
| Detection limit: | 70 pg/mL (2 fmol cortisol/well) |
| Dynamic range | 0.1 to 100 ng/mL |
| S/B | >30 |
| Cross-reactivity: | Cortisone 0.9% Corticosterone 7.0% Prednisolone 5.7% Cortisone 0.9% Progesterone 2.7% Prednisone <0.3% Testosterone <0.002% DHEA <0.02% Dexamethasone<0.0007% |
| Z': | 0.9 for 20 µl assay volumes |
Cortisol assessment on microsomes
The cortisol kit is intended to accurately measure hydrocortisone in complex samples such as serum, cells and microsome preparations. Figure 2 summarizes the results of a microsomal 11ß-HSD1 assay performed using HTRF cortisol reagents in low-volume 384-well plates. Known inhibitors of 11ß-HSD1, Carbenoxolone and Glycyrrhetinic acid, blocked the conversion of cortisone into cortisol. IC50 values were 0.33 and 0.31 µM respectively.
Microsomal 11ß-HSD1 (0.2mg/mL) was stimulated by NADPH (200µM) and cortisone (160nM) in a 384 low-volume plate. Various concentrations of the Glycyrrhetinic acid and Carbenoxolene inhibitors were added.
A standard curve with known cortisol concentrations was run concomitantly. Plates were incubated for 2 hours at 37°C and then a cortisol-d2 conjugate and Eu3+ Cryptate labeled anti-cortisol antibodies were added.
Plates were incubated at RT for 4 hours after reagent additions and emissions read at 620 and 665 nm.
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Manual Cortisol Kit / 62crtpeg-62crtpeh
62crtpeg-62crtpeh - Product Insert
Safety Data Sheet (DEU) Cortisol Kit / 62CRTPEG
62CRTPEG - Safety Data Sheet
Safety Data Sheet (ELL) Cortisol Kit / 62CRTPEG
62CRTPEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) Cortisol Kit / 62CRTPEG
62CRTPEG - Safety Data Sheet
Safety Data Sheet (ITA) Cortisol Kit / 62CRTPEG
62CRTPEG - Safety Data Sheet
Safety Data Sheet (SPA) Cortisol Kit / 62CRTPEG
62CRTPEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) Cortisol Kit / 62CRTPEG
62CRTPEG - Safety Data Sheet
Safety Data Sheet (ENG-US) Cortisol Kit / 62CRTPEG
62CRTPEG - Safety Data Sheet
Safety Data Sheet (DEU) Cortisol Kit / 62CRTPEH
62CRTPEH - Safety Data Sheet
Safety Data Sheet (ELL) Cortisol Kit / 62CRTPEH
62CRTPEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) Cortisol Kit / 62CRTPEH
62CRTPEH - Safety Data Sheet
Safety Data Sheet (ITA) Cortisol Kit / 62CRTPEH
62CRTPEH - Safety Data Sheet
Safety Data Sheet (SPA) Cortisol Kit / 62CRTPEH
62CRTPEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) Cortisol Kit / 62CRTPEH
62CRTPEH - Safety Data Sheet
Safety Data Sheet (ENG-US) Cortisol Kit / 62CRTPEH
62CRTPEH - Safety Data Sheet
Safety Data Sheet (DEU) Cortisol Kit / 62CRTPEJ
62CRTPEJ - Safety Data Sheet
Safety Data Sheet (ELL) Cortisol Kit / 62CRTPEJ
62CRTPEJ - Safety Data Sheet
Safety Data Sheet (FRA-FR) Cortisol Kit / 62CRTPEJ
62CRTPEJ - Safety Data Sheet
Safety Data Sheet (ITA) Cortisol Kit / 62CRTPEJ
62CRTPEJ - Safety Data Sheet
Safety Data Sheet (SPA) Cortisol Kit / 62CRTPEJ
62CRTPEJ - Safety Data Sheet
Safety Data Sheet (ENG-GB) Cortisol Kit / 62CRTPEJ
62CRTPEJ - Safety Data Sheet
Safety Data Sheet (ENG-US) Cortisol Kit / 62CRTPEJ
62CRTPEJ - Safety Data Sheet
Plate Reader Requirement
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