The CD32a cellular binding kit is designed for monitoring the binding of the IgG Fc region to the human CD32a receptor on live cells.
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  • Cellular binding assay
  • No-wash
  • All inclusive kit
The CD32a cellular binding kit is designed for monitoring the binding of the IgG Fc region to the human CD32a receptor on live cells.
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In stock

Overview

The CD32a (FcgRIIa, H131) cellular binding assay is a simple and robust test to monitor binding of the IgG Fc region to the human CD32a receptorin live cells and to select the most potent antibody for use in Antibody-Dependent Cell-Phagocytosis (ADCP). Two separate CD32a assays are available to specifically target the H131 or R131 variants. These assays, based on our innovative Tag-lite technology, can easily be automated to create a high-throughput solution for predicting ADCP.

Assay principle

The CD32a receptor cellular binding assay is a competitive assay involving HEK293 cells expressing a specific CD32a variant conjugated to Lumi4-terbium Cryptate, and human IgG1 labelled with d2. The unlabelled antibody or Fc tagged drug competes with the d2 labelled IgG1 for binding to the receptor, resulting in a decrease of FRET signal.

Assay protocol

The CD32a receptor cellular binding assays (FcgIIa H131 or R131) present only 1 incubation step after reagents distribution. Lumi4-terbium Cryptate pre-labelled cells are dispensed, followed by the addition of IgG1 labelled with d2 and the sample. After 2h incubation at RT, the signal can be read.
Product Insert CD32a (H131) Kit / 6FRAHPAG-6FRAHPAE-6FRAHPAF

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