The CD32b cellular binding kit is designed for monitoring the binding of the IgG Fc region to the human CD32b receptor on live cells.
See more
  • Cellular binding assay Cellular binding assay
  • No-wash No-wash
  • All inclusive kit All inclusive kit
The CD32b cellular binding kit is designed for monitoring the binding of the IgG Fc region to the human CD32b receptor on live cells.
-
In stock

Overview

The CD32b (FcgRIIb) cellular binding assay is a simple and robust test to monitor binding of the IgG Fc region to the human CD32b receptor in live cells. The CD32b receptor negatively modulates the Antibody-Dependent Cell-Phagocytosis (ADCP) pathway. This assay, based on our innovative Tag-lite technology, can easily be automated to create a high-throughput solution for predicting ADCP.

Benefits

  • FAST ANTIBODY SELECTION
  • ROBUSTNESS
  • LOW SAMPLE CONSUMPTION

Assay principle

The CD32b receptor cellular binding assay is a competitive assay involving HEK293 cells expressing a specific CD32b variant conjugated to Lumi4-terbium Cryptate, and human IgG1 labelled with d2. The unlabelled antibody or Fc tagged drug competes with the d2 labelled IgG1 for binding to the receptor, resulting in a decrease of FRET signal.
CD32b cellular binding assay protocol

Assay protocol

The CD32b receptor cellular binding assays (FcgIIb) present only 1 incubation step after reagents distribution. Lumi4-terbium Cryptate pre-labelled cells are dispensed, followed by the addition of IgG1 labelled with d2 and the sample. After 2h incubation at RT, the signal can be read.
CD32b cellular binding assay protocol
Product Insert CD32b Kit / 6FR2BPAG-6FR2BPAE-6FR2BPAF

6FR2BPAG-6FR2BPAE-6FR2BPAF - Product Insert

Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

tab6