The CD64 cellular binding kit is designed for monitoring the binding of the IgG Fc region to the human CD64 receptor on live cells.
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  • Cellular binding assay
  • No-wash
  • All inclusive kit
The CD64 cellular binding kit is designed for monitoring the binding of the IgG Fc region to the human CD64 receptor on live cells.
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In stock

Overview

The CD64 (FcgRI) cellular binding assay is a simple and robust test to monitor binding of the IgG Fc region to the human CD64 receptor in live cells and to select the most potent antibody for use in protective functions of the immune system. This assay, based on our innovative Tag-lite technology, can easily be automated to create a high-throughput solution for predicting phagocytosis and immune cell activation.

Assay principle

The CD64 receptor cellular binding assay is a competitive assay involving HEK293 cells expressing a specific CD64 variant conjugated to Lumi4-terbium Cryptate, and human IgG1 labelled with d2. The unlabelled antibody or Fc tagged drug competes with the d2 labelled IgG1 for binding to the receptor, resulting in a decrease of FRET signal.

Assay protocol

The CD64 receptor cellular binding assays (FcgI) present only 1 incubation step after reagents distribution. Lumi4-terbium Cryptate pre-labelled cells are dispensed, followed by the addition of IgG1 labelled with d2 and the sample. After 2h incubation at RT, the signal can be read.
Product Insert CD64 Kit / 6FC64PAG-6FC64PAE-6FC64PAF

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