Human CCL2 (MCP1) kit

The Human CCL2 (MCP1) kit is designed for the quantification of human CCL2 release in cell supernatant.
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  • No-wash
  • Low sample consumption
  • Under 2h bench time
The Human CCL2 (MCP1) kit is designed for the quantification of human CCL2 release in cell supernatant.
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In stock

Overview

Also called MCP-1 (monocyte chemoattractant protein 1), CCL2 is a chemokine mainly secreted by monocytes, macrophages, and dendritic cells to attract monocytes, basophils, and T cells. CCL2 is involved in pathologies like psoriasis or atherosclerosis. In the brain, CCL2 is released by neurons, astrocytes, and microglia, and is part of the neuro-inflammatory response observed in ischemia or Alzheimer's disease. CCL2 is also secreted by adipocytes and can interfere with insulin signaling, thus suggesting a role in the inflammation linked to diabetes.

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.

Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your CCL2 analysis.

Technical specifications of human CCL2 kit

Sample size16 µL
Final assay volume20 µL
Kit componentsLyophilized standard, frozen detection antibodies, buffers & protocol.
LOD & LOQ (in Diluent)20 pg/mL & 90 pg/mL
Range90 - 3,000pg/mL
Time to resultON at RT
Calibration NIBSC (92/794) value (IU/mL) = 0,9 x HTRF hCCL2 value (pg/mL)
LOD & LOQ (in Diluent)20 pg/mL & 90 pg/mL
SpeciesHuman only

Intra and inter assay

Sample Mean [CCL2] (pg/mL) CV
1 100 10%
2 1500 7%
3 2750 7%
  Mean CV 8%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

 Inter assay (n=4)

Sample[CCL2] (pg/mL)Mean (delta R)CV
11213352%
243710963%
3157930302%


Mean CV2%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

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