The HTRF human IL1 alpha kit is designed for the quantification of human IL1 alpha release in cell supernatant.

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  • No-wash No-wash
  • Low sample consumption Low sample consumption
  • Under 2h bench time Under 2h bench time

The HTRF human IL1 alpha kit is designed for the quantification of human IL1 alpha release in cell supernatant.

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Overview

Also known as hematopoietin 1, IL1 alpha is a pro-inflammatory cytokine mainly produced by activated macrophages, and epithelial and endothelial cells. Its main role is the maintenance and protection of the skin's barrier function. In response to infection, IL1 alpha stimulates fibroblast proliferation, increases blood neutrophils, and activates lymphocyte proliferation.


Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human IL-1 alpha AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.



Benefits

  • SCALABLE FROM 96 TO 1536WELLS
  • PANEL QUANTIFICATION

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
IL1 alpha kit assay principle

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.

Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL1 alpha analysis.

Technical specifications of human IL1 alpha kit

Sample size16 µL
Final assay volume20 µL
Kit componentsLyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent)8 pg/mL &20.5 pg/mL
Range20.5 – 5,000 pg/mL
Time to result2h at RT
CalibrationNIBSC (86/632) value (IU/mL) = 10 x HTRF hIL1a value (pg/mL)
SpeciesHuman only

Intra and inter assay

Intra-assay (n=24)

SampleMean [IL1a] (pg/mL)CV
15011%
225002%
345004%

Mean CV6%

Each of the 3 samples was measured 24 times, and % CV iwas calculated for each sample.

Inter-assay (n=4)

Sample[IL1a] (pg/mL)Mean (delta R)CV
1782466%
241112987%
3217459647%


Mean CV7%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

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