EPIgeneous Binding Domain kit B
- High sensitivity
- Good DMSO tolerance
The EPIgeneous Binding Domain kit series provides a simple biochemical approach to study epigenetic reader domain interactions with modified histones. All kits are based on a GST-tagged binding domain / biotin-coupled Histone peptide format, and can be run using the same add-and-read single plate protocol.
Binding Domain kit B has been successfully validated on a wide variety of readers (18), including some important therapeutic epigenetic targets.
The validation of the EPIgeneous binding Domain B assay was performed using the Tudor Domain UHRF1 binding to [Lys(9)Me3] H3(1-21) peptide. The GST-UHRF1 concentration was fixed at 5 nM while the peptide-biotin was serially diluted.
The HTRF signal was proportional to the specific interaction measured between GST-UHRF1 and peptide-biotin.
The 2nM Kd value was determined from this experiment using a two-site specific binding regression. A slight shift of apparent Kd is observed when DMSO% increases.
Robust assay tools to decipher key epigenetic interactions - Flyers
Enabling epigenetics studies from HTS to SAR: a novel HTRF® platform to identify and characterize reader domain inhibitors
Case study: BRD4 bromodomain - Posters
BMG Labtech - Application Notes