EPIgeneous H3K36Me2 cellular kit
- High sensitivity
This cellular assay has been developed with optimized reagents and protocols for the direct detection of endogenous levels of H3K36Me2. This HTRF® format uses a very specific antibody pair combined with a highly efficient nucleus extraction which enables the assay to be used with a variety of cell backgrounds, and for the study of important methyltransferases and demethylases targets, such as JMJD2A. The H3K36Me2 assay can be used for adherent or suspension cells, primary or secondary screening and inhibitor studies.
HTRF detection of H3K36Me2 mark with the two plate protocol in two cell lines: HeLa and U2OS. Cells were seeded at various densities in a 96-well plate and incubated for 24h before lysis without medium. Detection of H3K36Me2 was performed in a 384w white plate on a BMG Pherastar FS Flash lamp reader. For both cell lines, a hook effect was observed for the 30,000 cells per well concentration. For the same cellular concentration, U2OS showed an increase level of dimethylation on H3K36.
EPIgeneous cellular assays for measuring epigenetic modifications - Flyers
EPIgeneous Cell Based Assay Lysis Buffer Guide - Technical Notes