No-wash kit to quantify released Human IL17
SIRT 1 kit
- Low enzyme consumption
SIRT1 belongs to class III of the silent information regulator 2 (Sir2) family of proteins, which are histone/protein deacetylases (sirtuins). The use of SIRT1 modulators has been described as a potential therapeutic solution for treating multiple disease states including cancer, diabetes, aging, and neurodegenerative, metabolic or cardiovascular diseases. This rapid, simple assay enables the discrimination of polyphenolic compounds and can be used for high throughput screening to identify both inhibitors and activators of SIRT1 activity.
Z' values for different SIRT1 concentrations in twenty-four replicates were run using 4 selected SIRT1 concentrations: 0 mU of enzyme (no deacetylation), 100 mU of enzyme (100% deacetylation), 10 mU and 20 mU corresponding to the EC50 and EC80 respectively.
Titration curves were performed using 3 classes of compounds: two polyphenolic compounds (resveratrol and piceatannol), two non-polyphenolic compounds (activator1 and activator2 and one reference inhibitor (nicotinamide)
PGE2 quantification in cells and supernatants
Human IgG Lambda quantification for bioprocess applications
CD64 (FcgRI) cellular binding assay
CD32a (FcgRIIa, H131) cellular binding assay