A cell-based assay for histone H3K4Me2 epigenetic detection
Total H3 cellular kit
- High sensitivity
This cellular assay is for the direct detection of endogenous levels of Total H3 (histone 3) in cells. The assay can be used for adherent cells or suspension cells, primary or secondary screening, inhibitor studies and normalization studies of both methyltransferases and demethylases. The total H3 kit has been validated with Cisbios EPIgeneous H3K27ME3 and H3K36ME2 kits for normalization studies and be applied to the same assay lysates.
HTRF detection of H3K27Me3 and total Histone H3 in SU-DHL6 and OCI LY 19 cell lines using a two plate protocol. The test was run to assess the specificity of the acting compound.
Cells were seeded at 20.000cells per well in 96 well plate and treated for 72h with selective EZH2 inhibitor GSK126 (10µM down to 13.7nM diluted in medium).
The compound tested here showed no effect on Total Histone H3 below 3.33µM, whereas it fully inhibited the signal on H3K27Me3. 10µM of GSK126 clearly creates an inhibition on Total H3 detection.
EPIgeneous cellular assays for measuring epigenetic modifications - Flyers
EPIgeneous Cell Based Assay Lysis Buffer Guide - Technical Notes