A2AR expressing cells labeled with Terbium

Tag-lite cells transiently expressing the A2A receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
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  • Fully functional cell line
  • Transient expression
  • Ready for binding
Tag-lite cells transiently expressing the A2A receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
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In stock

Overview

One of four adenosine receptors found in the brain, A2A is a GPCR. A2A receptor is thought to play a role in acute and chronic neurodegenerative disorders such as cerebral ischemia or Parkinson's disease. A2A may also be involved in seizures and sleep disorders.

Cells expressing the A2A receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.

Assay principle

Running your A2A receptor binding assay using Tag-lite is as easy as it can gets. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.

Watch this video explaining how to run a saturation binding assay using Tag-lite.

Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.

Watch this video explaining how to run a competitive binding assay using Tag-lite.

Kd and Ki validation

Examples of data obtained using A2A labeled cells and their matching fluorescent ligand (L0058RED). Results may vary from one HTRF® compatible reader to another.

Evaluation of a Tag-lite binding assay for a class B receptor

In collaboration with Boehringer Ingelheim - Scientific Presentations

Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery

In collaboration with Bayer - Scientific Presentations

Tag-lite binding assays

benefit from them to move away from radioactivity - Flyers

HTRF, a powerful assay platform for Immune Checkpoint Studies

Binding Assays to measure therapeutic antitumor antibodies - Posters

Understanding GPCRs is the key to improved DD

In collaboration with GEN - Guides

Tag-lite, the easiest solution for studying GPCR-ligands interactions

The gold standard technology for receptor binding studies - Videos

Protocole A2A LabCell / C1TT1A2A

C1TT1A2A Lot 14 - Product Insert

Protocole A2A LabCell / C1TT1A2A

C1TT1A2A Lot 16 - Product Insert

Product Insert A2A LabCell / C1TT1A2A-05-2

C1TT1A2A-05-2 - Product Insert

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

Kinetic adenosine receptor family binding assays

Case study about kinetic binding characterization provided by the combination of Cisbio and Excellerate bioscience expertise. - Application Notes

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