Adenosine A1R fluorescent probe

This Adenosine A1 receptor red antagonist is a XAC derivative labeled with a red emitting HTRF fluorescent probe.

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  • Non-radioactive
  • No-wash
  • Cell-based
  • Equilibrium & Kinetic binding compatible

This Adenosine A1 receptor red antagonist is a XAC derivative labeled with a red emitting HTRF fluorescent probe.

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In stock

Overview

Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.

This Adenosine A1 receptor red antagonist is a XAC derivative labeled with a red emitting HTRF fluorescent probe.

This ligand is best suited for use in the Adenosine A1 receptor binding assay.

Benefits

  • FLUORESCENTLY LABELED LIGAND
  • HIGHLY STABLE
  • VALIDATED IN BINDING

Assay principle

Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells (C1TT1A1) into each well, followed by 5 µL of labeled ligand (L0067RED) and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells, and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.

Watch this video explaining how to run a saturation binding assay using Tag-lite.


Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.

Watch this video explaining how to run a competitive binding assay using Tag-lite.
Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

Evaluation of a Tag-lite binding assay for a class B receptor

In collaboration with Boehringer Ingelheim - Scientific Presentations

Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery

In collaboration with Bayer - Scientific Presentations

Tag-lite binding assays

benefit from them to move away from radioactivity - Flyers

HTRF, a powerful assay platform for Immune Checkpoint Studies

Binding Assays to measure therapeutic antitumor antibodies - Posters

Understanding GPCRs is the key to improved DD

In collaboration with GEN - Guides

Tag-lite, the easiest solution for studying GPCR-ligands interactions

The gold standard technology for receptor binding studies - Videos

Kinetic adenosine receptor family binding assays

Case study about kinetic binding characterization provided by the combination of Cisbio and Excellerate bioscience expertise. - Application Notes

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

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