Bombesin BB2R expressing cells labeled with Terbium

Tag-lite cells transiently expressing the Bombesin BB2 receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
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  • Fully functional cell line
  • Transient expression
  • Ready for binding
Tag-lite cells transiently expressing the Bombesin BB2 receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
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In stock

Overview

Mammalian bombesin receptors comprise 3 subtypes: BB1, BB2, BB3. Activation of BB1 and BB2 receptors causes smooth-muscle contraction, appetite and feeding behavior changes.

Cells expressing the BB2 receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.

Assay principle

Running your BB2 receptor binding assay using Tag-lite is as easy as it can gets. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.

Watch this video explaining how to run a saturation binding assay using Tag-lite.

Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.

Watch this video explaining how to run a competitive binding assay using Tag-lite.

Kd and Ki validation

Examples of data obtained using Bombesin BB2 labeled cells and their matching fluorescent ligand (L0019RED). Bombesin was used as reference ligand. Results may vary from one HTRF® compatible reader to another.

Evaluation of a Tag-lite binding assay for a class B receptor

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Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery

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Tag-lite binding assays

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Product Insert BB2 LabCell / C1TT1BB2

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Cisbio Product Catalog 2019

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Safety Data Sheet BB2 LabCell / C1TT1BB2

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