Optimal performance when applied to Gs activation or inhibition
cAMP Gi kit
This HTRF kit detects changes of cAMP accumulation in response to Gi coupled GPCR activation or inhibition
- High sensitivity
- Accurate pharmacology
Based on HTRF technology, the kit contains all the reagents needed to quantify cAMP, ensuring that you benefit from a complete tool for your needs.
Cisbio's cAMP kits are best suited for measuring cAMP modulations in response to Gi-activation, leveraging an optimized combination of robustness and sensitivity.
|S/B||IC10 nM||IC50 nM||IC90 nM|
|cAMP - Gi||>31||0.8||3.0||12|
The cAMP Gi kit allows enhanced performances for Gi coupled receptor assessment.
The data below shows the pharmacological validation of the Gi kit for the stably expressing delta opioid receptor cell line. Agonists and antagonists were well characterized, showing potency in agreement with the literature.
CHO cells stably expressing the delta opioid receptor (DOR) at 6000 cells/well were treated with the SNC-162, a reference agonist, for 45 min.
SNC-162 was well characterized, displaying the right potency in good correlation with the literature.
DOR Agonist SNC-162
|cAMP Gi||cAMP Gs dynamic|
|Optimal Cell density / well||6000||12000|
CHO cells stably expressing the delta opioid receptor (DOR) at 6000 cells/well were treated with the SNC-162 agonist for 45 min at the EC90 value (10 nM), which is the optimal concentration to use for the antagonist mode experiment.
Fitting the serial dilutions of the Naltrindole and the Naloxone antagonists enabled IC50 calculation of the compounds (potency), which was in perfect correlation with the literature.
|DOR Antagonists||cAMP Gi||cAMP Gs dynamic|
|Optimal Cells density / well||6000||6000||12000||12000|
Can HTRF technology answer to different scientific questions? An academic with industrial standards point of view
In collaboration with CNRS - Scientific Presentations
Structure, function and pharmacology of G protein-coupled receptors: State-of-the-art and future challenges
In collaboration with the Faculty of health and medical sciences Denmark - Scientific Presentations
Identification and characterization of allosteric modulators of GPCRs: The utility of HTRF and incorporation into generalised screening strategies
In collaboration with MRC technology - Scientific Presentations
Novel Functional Assay Approaches for GPCR Ligand Discovery. Comparison of three different technologies to assess slow acting agonist activity. FLIPR, CellKey and IP-One HTRF
In collaboration with Novartis - Scientific Presentations
In collaboration with Schering AG - Scientific Presentations
HTRF products and supporting scientific content to investifgate GPCRs - Flyers
In collaboration with Amylin Pharmaceuticals Inc., Biocon Limited - Posters
High Throughput Tag-Lite® Cell-based Functional and Surface Binding Assays on the SpectraMax®Paradigm Plate Reader Platform
In collaboration with Molecular Devices - Posters
Identification anad pharmacological characterisation of novel positive allosteric modulators of Melanocortin 2 receptors
In collaboration with MRC - Posters
In collaboration with Sanofi - Posters
In collaboration with College of Science, King Saud University, University of Western Australia - Posters
In collaboration with Mollecular Devices - Posters
In collaboration with Chinese Academy of Sciences - Posters
Featuring a panel of experts - Videos
Optimizing cAMP assays has never been easier - Guides
Featuring a panel of experts - Videos
4 GPCR challenges, 1 solution - Posters
Written by a panel of experts - Guides
Identification and characterization of novel positive allosteric modulators of the Dopamine D1 Receptor
Identifying therapeutic candidates for Schizophrenia through HTS - Posters
Get the brochure about technology comparison. - Brochures
Dedicated to the study of Gs coupled GPCR
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