CCK2R expressing cells labeled with Terbium

Tag-lite cells transiently expressing the CCK2 receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
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  • Fully functional cell line
  • Transient expression
  • Ready for binding
Tag-lite cells transiently expressing the CCK2 receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
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In stock

Overview

Cholecystokinin receptors are also called CCK-A (or CCK-1) and CCK-B. CCK2 is also known as the gastrin receptor. Cholecystokinin receptor hypersensitivity has been implicated in Parkinson's disease.

Cells expressing the CCK2 receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.

Assay principle

Running your CCK2 receptor binding assay using Tag-lite is as easy as it can gets. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.

Watch this video explaining how to run a saturation binding assay using Tag-lite.

Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.

Watch this video explaining how to run a competitive binding assay using Tag-lite.

Kd and Ki validation

Examples of data obtained using CCK2 labeled cells and their matching fluorescent ligand. Results may vary from one HTRF® compatible reader to another.
Evaluation of a Tag-lite binding assay for a class B receptor

In collaboration with Boehringer Ingelheim - Scientific Presentations

Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery

In collaboration with Bayer - Scientific Presentations

Tag-lite binding assays

benefit from them to move away from radioactivity - Flyers

Understanding GPCRs is the key to improved DD

In collaboration with GEN - Guides

Tag-lite, the easiest solution for studying GPCR-ligands interactions

The gold standard technology for receptor binding studies - Videos

Kinetics of the ß2 Adrenergic receptor binding and signaling

A look at the kinetics of cAMP production in cells - Application Notes

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

Safety Data Sheet CCK2 LabCell / C1TT1CCK2

C1TT1CCK2 - Safety Data Sheet

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