This immune checkpoint assay is designed to identify human PD1/PD-L1 checkpoint inhibitors.
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  • All inclusive kit All inclusive kit
  • Scalable from 96 to 1536 format Scalable from 96 to 1536 format
  • Validated with reference Ab Validated with reference Ab
This immune checkpoint assay is designed to identify human PD1/PD-L1 checkpoint inhibitors.
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Overview

Programmed cell death protein 1 (PD1) is an immune checkpoint receptor that regulates T cell response. Its ligand, programmed death-ligand 1 (PD-L1), is commonly over-expressed on the tumor cell surface. When PD1 is bound to PD-L1, T cell response is suppressed, contributing to tumor immune resistance. Checkpoint inhibitors blocking PD1/PD-L1 complex formation are generating considerable interest in cancer immunotherapy.

Benefits

  • NO WASH BIOCHEMICAL FORMAT
  • EASY INHIBITOR CHARACTERIZATION
  • HTS COMPATIBLE

Assay principle

This PD1/PD-L1 kit includes human recombinant immune checkpoint partners tagged for detection (PD1 and PD-L1), a reaction buffer, and labelled anti-tag antibodies for HTRF detection. Without treatment, PD1 binds to PD-L1, and the binding of each detection antibody to its tag target generates an HTRF signal. In the presence of a compound or an anti-human blocking antibody, the PD1/PD-L1 interaction is disrupted and the HTRF signal decreases.
PD1/PD-L1 kit assay principle

Inhibitory effects of PD1/PD-L1 blockers

The inhibitory effects of small molecules, human and mouse anti-human PD1, and anti-human PD-L1 were tested at 5 nM PD-L1 and 50 nM PD1. An assay window of 10-fold was obtained.
Inhibitory effects of PD1/PD-L1 blockers
PD1PD-L1 binding assay

Assay protocol and validations - Technical Notes

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HTRF, a powerful assay platform for Immune Checkpoint Studies

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Product Insert PD1/PD-L1 Kit / 64ICP01PEG-64ICP01PEH

64ICP01PEG-64ICP01PEH - Product Insert

STING HTRF offer to bridge innate and adaptive immunity

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HTRF PPI your dream assay served on a plate

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Deciphering low- and high affinity interactions - Application Notes

Nuclear receptor ligand identification with HTRF

Monitoring nuclear receptor binding with HTRF assays - Application Notes

HTRF addresses large protein-protein interaction complexes

Challenge large complexes with HTRF assays - Application Notes

A brief history of Protein-Protein Interactions

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Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

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Functional response to PD1 blockade rapidly assessed through HTRF IFNγ quantification

For researcher working on checkpoint inhibitors - Application Notes

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

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