HTRF KinEASE-STK S1 kit
- Low enzyme consumption
The HTRF KinEASE kit series provides a simple biochemical approach to study kinase activity, screen for inhibitors and characterize them. Three STK and one TK kits, offer a semi-universal method for assessing phosphorylation on Serine/Threonine and Tyrosine residues, respectively. The HTRF KinEASE STK S1 assay has been validated on 56 Serine/Threonine kinases. The assay validation section of this page lists them and provides technical tips for setup. The kit series also include a Discovery kit to help determine which of three STK kits is appropriate when working with a non-validated enzyme.
As shown here, all HTRF KinEASE assays work the same way and involve two steps:
Step 1: Kinase reaction The kinase is incubated in the presence or absence of compounds and the appropriate substrate. The supplemented enzymatic buffer is added in the kinase buffer for tyr kinases only. ATP is added to start the reaction.
Step 2: Detection Eu3+ cryptate and XL665 conjugates are added. The detection buffer contains EDTA to stop the enzymatic reaction.
Enzyme validation - Technical Notes
A universal expanded platform to address Serine/Threonine & Tyrosine kinases - Application Notes
Development of an HTRF-based High Throughput assay for the identification of natural ligands for AMP-activated protein kinase
In collaboration with Nestlé Institute of Health Sciences - Posters
Measuring kinase activity under any conditions - Application Notes
62ST1PEB-62ST1PEC-62ST1PEJ - Product Insert