HTRF KinEASE-TK kit
- Low enzyme consumption
The HTRF KinEASE kit series provides a simple biochemical approach to study kinase activity, screen for inhibitors and characterize them. Three STK and one TK kits offer a semi-universal method for assessing phosphorylation on Serine/Threonine and Tyrosine residues, respectively. The HTRF KinEASE TK assay has been validated on 71 Tyrosine kinases, using a single generic substrate. The assay validation section of this page lists them and provides technical tips for setup.
As shown here, all HTRF KinEASE assays work the same way and involve two steps:
Step 1: Kinase reaction The kinase is incubated in the presence or absence of compounds and the appropriate substrate. The supplemented enzymatic buffer is added in the kinase buffer for tyr kinases only. ATP is added to start the reaction.
Step 2: Detection Eu-cryptate and XL665 conjugates are added. The detection buffer contains EDTA to stop the enzymatic reaction.
A universal expanded platform to address Serine/Threonine & Tyrosine kinases - Application Notes
Measuring kinase activity under any conditions - Application Notes
62TK0PEB-62TK0PEC-62TK0PEJ - Product Insert