A method for determining enzyme kinetic parameters (Km, Kcat) using ...
MAb Anti-phosphop44/42-Eu cryptate
- High affinity
The Anti-phospho p44/42-Eu cryptate is a labeled monoclonal antibody enabling the rapid detection of p44/42 MAPK only when phosphorylated at Thr202 & Tyr204. It does not cross-react with phosphorylated forms of SAP/JNK or p38 MAPK. It does not detect the non-phosphorylated p44/42. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSHO-P44/42 (THR202/TYR204) SPECIFIC
61P10KAE-61P10KAZ-61P10KAY - Product Insert
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