MAb PY20-Eu cryptate enables the rapid detection of phosphorylation of peptides or proteins on Tyrosine in a biochemical kinase assay format.
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  • No-wash
  • Ease-of-use
  • High affinity
MAb PY20-Eu cryptate enables the rapid detection of phosphorylation of peptides or proteins on Tyrosine in a biochemical kinase assay format.
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Overview

PY20-Eu cryptate is a labeled mouse monoclonal IgG2b. It shows to react specifically with phosphorylated tyrosine, both as free amino acid or when conjugated to carriers. No cross-reactivity has been observed with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP. This allows the construction of a highly specific biochemical Tyrosine kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. HTRF allows to perform assay at physiological ATP concentrations.

Assay principle

HTRF Tyrosine kinase assay use typically a combination of a Europium-cryptate labeled anti-phosphotyrosine antibody, SA-XL665 and a biotinylated substrate. The signal is proportional to the concentration of phospho-residues. Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.

Assay protocol

HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and MAb PY20-Eu cryptate prepared in a buffer containing EDTA to stop the enzymatic reaction.
Open R&D: Sanofi Access Platform

In collaboration with Sanofi - Scientific Presentations

Experience the freedom of building your own assay

HTRF kinase activity toolbox reagents - Flyers

Product Insert PY20-Eu / 61Y20KLA-61Y20KLB

61Y20KLA-61Y20KLB - Product Insert

Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

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Learn about cutting edge kinase research in this free white paper - Guides

Safety Data Sheet PY20-Eu / 61Y20KLA

61Y20KLA - Safety Data Sheet

Safety Data Sheet PY20-Eu / 61Y20KLB

61Y20KLB - Safety Data Sheet

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