HTRF kinase assay allows high concentrations of ATP, making possible...
MAb PY20-Eu cryptate
- High affinity
PY20-Eu cryptate is a labeled mouse monoclonal IgG2b. It shows to react specifically with phosphorylated tyrosine, both as free amino acid or when conjugated to carriers. No cross-reactivity has been observed with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP. This allows the construction of a highly specific biochemical Tyrosine kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. HTRF allows to perform assay at physiological ATP concentrations.
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSHO-(TYR) SPECIFIC
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A review of applications to the study of molecular interactions invo...
A comparison of the 2 methods for tyrosine kinases