A method for determining enzyme kinetic parameters (Km, Kcat) using ...
PAb Anti-phospho ATF2-Eu cryptate
- High affinity
The Anti-phospho ATF2-Eu cryptate is a labeled polyclonal antibody enabling the detection of ATF2 when singly phosphorylated at Ser 71 or dually phosphorylated at Thr69/71. It does not detect the non-phosphorylated ATF2. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSHO-ATF2 (THR71) SPECIFIC
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