A method for determining enzyme kinetic parameters (Km, Kcat) using ...
PAb Anti-phospho MEK1/2-Eu cryptate
- High affinity
The Anti-phospho MEK1/2 substrate-Eu cryptate is a labeled polyclonal antibody enabling the detection of MEK1/2 when phosphorylated at Ser217/221. It recognizes singly phosphorylated MEK1/2 at Ser 217 but not MEK1/2 singly phosphorylated at Ser 221. It does not detect the non-phosphorylated MEK1/2. This allows the construction of a specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. HTRF allows to perform assay at physiological ATP concentrations.
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSHO-MEK1/2 (SER217/221) SPECIFIC
|Assay Format||Apparent ATP Km(µM)|
|COT/MBP protein direct33P||384 ± 55|
|COT/MEK protein direct HTRF||279 ± 40|
|COT/MEK peptide direct HTRF||268 ± 21|
61P17KAE-61P17KAZ-61P17KAY - Product Insert
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