PolyGT;Biotin is a substrate for tyrosine;specific protein kinases
The Anti-phospho PKA-Eu cryptate is a labeled polyclonal antibody enabling the detection of peptides and proteins containing a phosphorylated Ser/Thr residue with Arginine in the 3 position. It does not detect the non-phosphorylated PKA. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrates can be used, from small synthetic peptides, to natural substrates for a high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.
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How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes