A method for determining enzyme kinetic parameters (Km, Kcat) using ...
PAb Anti-phospho PKA substrate-Eu cryptate
- High affinity
The Anti-phospho PKA-Eu cryptate is a labeled polyclonal antibody enabling the detection of peptides and proteins containing a phosphorylated Ser/Thr residue with Arginine in the 3 position. It does not detect the non-phosphorylated PKA. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrates can be used, from small synthetic peptides, to natural substrates for a high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSHO-(SER/THR) SPECIFIC
Learn about cutting edge kinase research in this free white paper - Guides
Get the brochure about technology comparison. - Brochures