Europium cryptate Labeling kit

Europium cryptate labeling kit tailored for biomolecule labeling and straightforward development of time-resolved fluorescence resonance energy transfer assays.
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Europium cryptate labeling kit tailored for biomolecule labeling and straightforward development of time-resolved fluorescence resonance energy transfer assays.
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Overview

The Cryptate labeling kit is suitable for quick, easy labeling of peptides, proteins, and oligonucleotides with Europium cryptate. This kit is designed for very small-volume fast labeling needs, such as determining the best antibody among several antibodies available for HTRFassay development.

The N-hydroxysuccinimide activated Europium-Trisbipyridine (TBP) supplied reacts with primary amines carried by the biomolecules to label, for simplified conjugation.

The kit includes all the materials necessary for the labeling procedure (except dialysis and concentration devices).

Benefits

  • SPECIFIC NH2 LABELING
  • NO CHROMATOGRAPHIC EQUIPMENT NEEDED
  • FAST LABELING PROCEDURE < 1 H

Assay principle

The labeling procedure is performed under very mild conditions. As shown here, the process includes an incubation step with the desiccated dye, followed by purification on a disposable column. Both the purification column and the buffer for purifying the conjugate are included in the kit, as well as a calibrator to define the ideal working dilution of the labeled material in HTRF assays. Depending on the molecular weight of the target molecule and the nature of the dye, each kit enables the labeling of 5-25 µg (protein-MW 15 kDa) to 50-250 µg (antibody-MW 150 kDa).
assay principle of the labeling of a molecule using HTRF Europium cryptate Labeling kit

Assay protocol

The labeling procedure is described on the right. 50 µL of the targeted molecule (6.67 µM) in phosphate buffer pH 8.0 are added to the Europium cryptate vial. After an incubation of 15 min at RT, labeled protein is eluted on an equilibrated column. After having discarded 700 µL of elution buffer, the labeled molecule is collected in 400µL fraction
assay protocol of the labeling of a molecule using HTRF Europium cryptate labeling kit¶
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