The GST check kit enables the rapid detection of GST tagged fusion proteins.
See more
  • No-wash
  • Ready-to-use
  • Ease-of-use
The GST check kit enables the rapid detection of GST tagged fusion proteins.
-
In stock

Overview

The kit enables the rapid detection of GST tagged fusion proteins at each step of protein production and purification. It can either be used to ensure that the GST motif of a fusion protein is accessible to the anti-GST antibody, or to approximate the concentration of a GST-tagged protein. Calibrated using the appropriate protein, the assay is very accurate and compatible with the SOPs. It can also be used to eliminate false positives.

Assay principle

As shown in the graph, GST peptide labeled with XL665 is detected by anti GST Cryptate conjugate (anti-tag Cryptate). The GST-tagged fusion protein (Tag fusion protein) to be ascertained competes with the GST-peptide-XL665 conjugate (Tag-XL665) for binding to the anti-GST-Cryptate conjugate. The specific signal is inversely proportional to the concentration of GST-tagged fusion protein.

Assay protocol

The example on the right describes the protocol using a 20 µL final assay volume. Dispense 10 µL of standard or of GST-tagged protein of interest into a well, with 5 µL of GST Cryptate conjugate and 5 µL of GST-XL665 conjugate. Incubate for 2 hours at RT and read.

Native GST standard curve using GST Check Kit

Detection limit: 1 ng/mL (average)
S/B 15
in 96- or 384-well formats

GST Check Kit performance on various GST-tagged proteins

The GST check kit enables the verification of tag accessibility on GST-tagged fusion proteins. The figure shows the dose response curves of different GST fusion proteins assessed using this kit. All six GST containing proteins were detected using our competitive immunoassay.
HTRF check kits for GST and 6His tag accessibility

Procedure and guidelines for assessing tagged proteins - Application Notes

Accelerate throughput without compromising on performance

HTRf® reagents for biotherapeutics - Flyers

HTRF PPI your dream assay served on a plate

Sandwiches aren't just for eating - Infographics

Best practices for pharmacological characterization of PPI inhibitors

Easy pharmacological characterization of PPI modulators. - Technical Notes

HTRF assays handle low- to high affinity protein-protein interactions

Deciphering low- and high affinity interactions - Application Notes

Nuclear receptor ligand identification with HTRF

Monitoring nuclear receptor binding with HTRF assays - Application Notes

HTRF addresses large protein-protein interaction complexes

Challenge large complexes with HTRF assays - Application Notes

A brief history of Protein-Protein Interactions

How well do you know PPI? - Infographics

Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

Addressing the interactome with protein-protein assays

Cover all PPIs with one approach - Brochures

tab6