NK1R stably expressing cells

This cell line stably expresses the NK1 receptor fused to a SNAP-Tag, and can be used in a Tag-lite application.
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  • Fully functional cell line
  • Stable expression
This cell line stably expresses the NK1 receptor fused to a SNAP-Tag, and can be used in a Tag-lite application.
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In stock

Overview

Tachykinin receptor 1 or NK1 receptor is a GPCR. NK1 receptors are found in the GI tract, where it is a component of the centrally mediated vomiting reflex.

This HEK293 stable cell line expresses the NK1 receptor fused to a SNAP-Tag. Labeled with Terbium, the cells can be used in a Tag-lite receptor binding protocol.

Benefits

  • STABLY TRANFECTED
  • HIGH PECENTAGE OF VIABILITY
  • FULLY FUNCTIONAL

Step 1 - Receptor labeling

SNAP-tag®, is a small fusion tag that covalently interacts with specific substrates. SNAP-tag allows specific and covalent labeling of any protein of interest. For more details, see the labeling procedure.

Watch this video describing how to label cell surface receptors using Tag-lite® technology.

Step 2 - Understand the assay principle

Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

Step 3 - Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.

Watch this video explaining how to run a saturation binding assay using Tag-lite.

Step 4 - Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.

Watch this video explaining how to run a competitive binding assay using Tag-lite.

Evaluation of a Tag-lite binding assay for a class B receptor

In collaboration with Boehringer Ingelheim - Scientific Presentations

Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery

In collaboration with Bayer - Scientific Presentations

Tag-lite binding assays

benefit from them to move away from radioactivity - Flyers

Understanding GPCRs is the key to improved DD

In collaboration with GEN - Guides

Tag-lite, the easiest solution for studying GPCR-ligands interactions

The gold standard technology for receptor binding studies - Videos

Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

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How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

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