Human Fc quantification for bioprocess applications
Human IgG kit
- Highly specific
- Faster and more convenient than ELISA
Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The human IgG kit enables a rapid quantification of human IgG in cell supernatant and the selection of the best producer clones. The assay is compatible with natural IgG as well as recombinant antibodies.
The figure below shows the human IgG kit calibration curve. The assay was performed in a 384-well small volume white microplate in a 20 µL total volume, using 10µL human IgG calibrator diluted in PBS 0.1 M and 5 µL of each conjugate. The two HTRF conjugates may be pre-mixed and added in a single dispensing step to further streamline the protocol. After 2h of incubation, the signal was read on a PHERAstarFS flash lamp.
|Detection limit||0.9 ng/mL|
|Assay range||0.9 to 2000 ng/mL|
|Incubation time||2h at RT|
|Specificity||IgG1, IgG2, IgG3, IgG4|
HTRf® reagents for biotherapeutics - Flyers