The Human CCL4 (MIP1 beta) kit is designed for the quantification of human CCL4 release in cell supernatant.
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  • No-wash
  • Low sample consumption
  • Under 2h bench time
The Human CCL4 (MIP1 beta) kit is designed for the quantification of human CCL4 release in cell supernatant.
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In stock

Overview

Also known as MIP-1beta (macrophage inflammatory protein 1 beta), CCL4 is a chemokine secreted by activated leukocytes, lymphocytes, and endothelial and muscle cells in response to microbial infection, ischemia, or asthma.

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases. To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page. Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your CCL4 analysis.

Technical specifications of human CCL20 kit

Sample size 16 µL
Final assay volume 20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent) 7 pg/mL &20 pg/mL
Range 20 – 5,000 pg/mL
Time to result 1h at RT
Species Human only

Intra and inter assay

Intra-assay (n=24)

Sample Mean [CCL4] (pg/mL) CV
1 36 9%
2 2263 5%
3 4280 5%
Mean CV 6%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample [CCL4] (pg/mL) Mean (delta R) CV
1 78 657 13%
2 411 2979 16%
3 2174 13278 15%
Mean CV 15%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

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